ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSSYDRRFAD PNSYRQRSGA PVGSSQPMDP SAAPYNPRYT GGGGGYGPSP VMAGDNSGYN 

        70         80         90        100        110        120 
RYPSFQPPSG GFSVGRGGGR GGYGQYGDRN GGGNWGGGGG RGGSSKRELD SVSLPKQNFG 

       130        140        150        160        170        180 
NLVHFEKNFY VESPTVQAMT EQDVAMYRTE RDISVEGRDV PKPMKMFQDA NFPDNILEAI 

       190        200        210        220        230        240 
AKLGFTEPTP IQAQGWPMAL KGRDLIGIAE TGSGKTLAYL LPALVHVSAQ PRLGQDDGPI 

       250        260        270        280        290        300 
VLILAPTREL AVQIQEESRK FGLRSGVRST CIYGGAPKGP QIRDLRRGVE IVIATPGRLI 

       310        320        330        340        350        360 
DMLECQHTNL KRVTYLVLDE ADRMLDMGFE PQIRKIVSQI RPDRQTLLWS ATWPREVETL 

       370        380        390        400        410        420 
ARQFLRDPYK AIIGSTDLKA NQSINQVIEI VPTPEKYNRL LTLLKQLMDG SKILIFVETK 

       430        440        450        460        470        480 
RGCDQVTRQL RMDGWPALAI HGDKTQSERD RVLAEFKSGR SPIMTATDVA ARGLDVKDIK 

       490        500        510        520        530        540 
CVVNYDFPNT LEDYIHRIGR TGRAGAKGMA FTFFTHDNAK FARELVKILQ EAGQVVPPTL 

       550        560        570        580        590 
SALVRSSGSG YGGSGGGRNF RPRGGGRGGG FGDKRSRSTS NFVPHGGKRT W 

References and documentation are available.

Number of amino acids: 591

Molecular weight: 64621.23

Theoretical pI: 9.66

Amino acid composition: 
Ala (A)  38	  6.4%
Arg (R)  52	  8.8%
Asn (N)  20	  3.4%
Asp (D)  34	  5.8%
Cys (C)   4	  0.7%
Gln (Q)  28	  4.7%
Glu (E)  26	  4.4%
Gly (G)  76	 12.9%
His (H)   7	  1.2%
Ile (I)  30	  5.1%
Leu (L)  43	  7.3%
Lys (K)  27	  4.6%
Met (M)  15	  2.5%
Phe (F)  22	  3.7%
Pro (P)  39	  6.6%
Ser (S)  39	  6.6%
Thr (T)  31	  5.2%
Trp (W)   6	  1.0%
Tyr (Y)  19	  3.2%
Val (V)  35	  5.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 60
Total number of positively charged residues (Arg + Lys): 79

Atomic composition:

Carbon      C	      2839
Hydrogen    H	      4500
Nitrogen    N	       842
Oxygen      O	       849
Sulfur      S	        19

Formula: C2839H4500N842O849S19
Total number of atoms: 9049

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    61560
Abs 0.1% (=1 g/l)   0.953, assuming all pairs of Cys residues form cystines


Ext. coefficient    61310
Abs 0.1% (=1 g/l)   0.949, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 45.25
This classifies the protein as unstable.



Aliphatic index: 71.78

Grand average of hydropathicity (GRAVY): -0.510