ProtParam
User-provided sequence:
10 20 30 40 50 60
MSTADQEQPK VVEATPEDGT ASSQKSTINA ENENTKQNQS MEPQETSKGT SNDTKDPDNG
70 80 90 100 110 120
EKNEEAAIDE NSNVEAAERK RKHISTDFSD DDLEKEEHND QSLQPTVENR ASKDRDSSAT
130 140 150 160 170 180
PSSRQELEEK LDRILKKPKV RRTRRDEDDL EQYLDEKILR LKDEMNIAAQ LDIDTLNKRI
190 200 210 220 230 240
ETGDTSLIAM QKVKLLPKVV SVLSKANLAD TILDNNLLQS VRIWLEPLPD GSLPSFEIQK
250 260 270 280 290 300
SLFAALNDLP VKTEHLKESG LGRVVIFYTK SKRVEAQLAR LAEKLIAEWT RPIIGASDNY
310 320 330 340 350 360
RDKRIMQLEF DSEKLRKKSV MDSAKNRKKK SKSGEDPTSR GSSVQTLYEQ AAARRNRAAA
370 380 390 400 410
PAQTTTDYKY APVSNLSAVP TNARAVGVGS TLNNSEMYKR LTSRLNKKHK
References and
documentation are available.
Number of amino acids: 410
Molecular weight: 46082.48
Theoretical pI: 6.97
Total number of negatively charged residues (Asp + Glu): 69
Total number of positively charged residues (Arg + Lys): 69
Atomic composition:
Carbon C 1971
Hydrogen H 3253
Nitrogen N 589
Oxygen O 666
Sulfur S 7
Formula: C
1971H
3253N
589O
666S
7
Total number of atoms: 6486
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 21430
Abs 0.1% (=1 g/l) 0.465
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 47.38
This classifies the protein as unstable.
Aliphatic index: 73.80
Grand average of hydropathicity (GRAVY): -0.970