ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSTDAEMAVY GKAAIYLRKP ERERIEAQSK PFDAKAACYV ADVKELYLKA LITKKDGAKV 

        70         80         90        100        110        120 
TVKVLDTEEE RTVKEDDVTP MNPPKFDKIE DMAMMTHLNE ASVLYNLKER YAAWMIYTYS 

       130        140        150        160        170        180 
GLFCATVNPY KWLPVYDSEV VNAYRGKKRM EAPPHIFSVS DNAFQFMLID RENQSVLITG 

       190        200        210        220        230        240 
ESGAGKTVNT KRVIQYFATI SVGGEKKRDT SKGSLEDQII AANPLLEAYG NAKTVRNDNS 

       250        260        270        280        290        300 
SRFGKFIRVH FGATGKLASA DIETYLLEKS RVTFQLPDER GYHIFYQMMT NHKPELIEMS 

       310        320        330        340        350        360 
LITTNPYDFP MCSMGQITVA SIDDKVELEA TDNAIDILGF TSDEKMSIYK MTGAVLHHGS 

       370        380        390        400        410        420 
MKFKQKQREE QAEPDGTEDA DKVAYLLGLN SADMLKALCY PRVKVGNEYV TKGQTVPQVN 

       430        440        450        460        470        480 
NSVTALAKSI YEKMFLWMVI RINEMLDTKQ ARQFFIGVLD IAGFEIFDFN TLEQLCINFT 

       490        500        510        520        530        540 
NEKLQQFFNH TMFVLEQEEY KKEGIVWEFI DFGMDLAACI ELIEKPMGIF SILEEECMFP 

       550        560        570        580        590        600 
KATDTSFKNK LYDQHLGKNK AFEKPKPAKG KAEAHFSLVH YAGTVDYNIA GWLDKNKDPL 

       610        620        630        640        650        660 
NESVVQLYQK SSVKLLPVLY PPVVEETGGK KGKKKGGSMQ TVSAAFRENL GKLMTNLRST 

       670        680        690        700        710        720 
HPHFVRCLIP NESKTPGLME NFLVIHQLRC NGVLEGIRIC RKGFPSRIPY ADFKQRYKVL 

       730        740        750        760        770        780 
NASVIPDGQF MDNKKASEKL LGSIDVDHDQ YRFGHTKVFF KAGLLGTLEE MRDEKLAALV 

       790        800        810        820        830        840 
TMTQALCRGY VMRKEFVKMM ERREAIYTIQ YNVRSFTNVK NWPWMKVYYK IKPMLKSAET 

       850        860        870        880        890        900 
EKELANMKEN YEKMKTDLAT ALAKKKELEE KMVSIVQEKN DLQLQVASES ENLSDAEERC 

       910        920        930        940        950        960 
EGLIKSKIQM EAKLKETSER LEDEEEINAE LTAKKRKLED ECSELKKDID DLELTLAKVE 

       970        980        990       1000       1010       1020 
KEKHATENKV KNLTEEMASQ DDSLAKLTKE KKALQEAHQQ TLDDLQAEED KVNTLTKAKT 

      1030       1040       1050       1060       1070       1080 
KLEQQVDDLE GSLEQEKKLR MDLERAKRKL EGDLKLAQES IMDLENDKQQ TDEKLKKKDF 

      1090       1100       1110       1120       1130       1140 
EISQLLSKIE DEQSLGAQLQ KKIKELQARI EELEEEIEAE RAARAKVEKQ RADLSRELEE 

      1150       1160       1170       1180       1190       1200 
ISERLEEAGG ATASQIEMNK KREAEFQKLR RDLEESTLQH EATAAALRKK QADSVAELGE 

      1210       1220       1230       1240       1250       1260 
QIDNLQRVKQ KLEKEKSEYK MEIDDLSSNM EAVAKAKGNL EKMCRTLEDQ LSELKTKNDE 

      1270       1280       1290       1300       1310       1320 
NVRQINDMSG QRARLLTENG EFTRQVEEKE ALVSQLTRGK QAFTQQIEEL KRQIEEEVKA 

      1330       1340       1350       1360       1370       1380 
KNALAHGVQS ARHDCDLLRE QFEEEQEAKA ELQRGMSKAN SEVAQWRSKY ETDAIQRTEE 

      1390       1400       1410       1420       1430       1440 
LEEAKKKLAQ RLQDAEEQIE AVNSKCASLE KTKQRLQSEV EDLMIDVERA NGLAANLDKK 

      1450       1460       1470       1480       1490       1500 
QRNFDKVLAE WKQKYEEGQA ELEGAQKEAR SLGTELFKMK NSYEESLDQL ETMKRENKNL 

      1510       1520       1530       1540       1550       1560 
QQEISDLTEQ IGETGKSIHE LEKAKKQVET EKSEIQTALE EAEGTLEHEE SKILRVQLEL 

      1570       1580       1590       1600       1610       1620 
NQVKGEVDRK LAEKDEEMEQ IKRNSQRVID SMQSTLDSEV RSRNDALRIK KKMEGDLNEM 

      1630       1640       1650       1660       1670       1680 
EIQLSHANRQ AAESQKQLRN VQAQLKDAQL HLDDAVRAQD DFKEQAAMVE RRNGLMMAEI 

      1690       1700       1710       1720       1730       1740 
EELRVALEQT ERSRKVAEQE LVDASERVGL LHSQNTSLMN TKKKLEADLV QIQGEVDDTV 

      1750       1760       1770       1780       1790       1800 
QEARNAEEKA KKAITDAAMM AEELKKEQDT SSHLERMKKN LEVAVKDLQH RLDEAENLAM 

      1810       1820       1830       1840       1850       1860 
KGGKKQLQKL ESRVRELEAE VEAEQRRGGD AVKGVRKYER RVKELTYQTE EDKKNVARLQ 

      1870       1880       1890       1900       1910       1920 
DLVDKLQLKV KGYKRQAEEA EEQANVHLSK CRKIQHELEE AEERADIAET QVNKLRVKSR 

      1930 
DIGKGKDAAE 

References and documentation are available.

Number of amino acids: 1930

Molecular weight: 221209.28

Theoretical pI: 5.50

Amino acid composition: 
Ala (A) 165	  8.5%
Arg (R) 101	  5.2%
Asn (N)  80	  4.1%
Asp (D) 111	  5.8%
Cys (C)  17	  0.9%
Gln (Q) 119	  6.2%
Glu (E) 250	 13.0%
Gly (G)  81	  4.2%
His (H)  30	  1.6%
Ile (I)  82	  4.2%
Leu (L) 187	  9.7%
Lys (K) 213	 11.0%
Met (M)  64	  3.3%
Phe (F)  51	  2.6%
Pro (P)  33	  1.7%
Ser (S)  96	  5.0%
Thr (T)  90	  4.7%
Trp (W)   9	  0.5%
Tyr (Y)  44	  2.3%
Val (V) 107	  5.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 361
Total number of positively charged residues (Arg + Lys): 314

Atomic composition:

Carbon      C	      9617
Hydrogen    H	     15654
Nitrogen    N	      2714
Oxygen      O	      3082
Sulfur      S	        81

Formula: C9617H15654N2714O3082S81
Total number of atoms: 31148

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   116060
Abs 0.1% (=1 g/l)   0.525, assuming all pairs of Cys residues form cystines


Ext. coefficient   115060
Abs 0.1% (=1 g/l)   0.520, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.22
This classifies the protein as unstable.



Aliphatic index: 78.98

Grand average of hydropathicity (GRAVY): -0.776