ProtParam
User-provided sequence:
10 20 30 40 50 60
MSTDFDRIYL NQSKFSGRFR IADSGLGWKI STSGGSAANQ ARKPFLLPAT ELSTVQWSRG
70 80 90 100 110 120
CRGYDLKINT KNQGVIQLDG FSQDDYNLIK NDFHRRFNIQ VEQREHSLRG WNWGKTDLAR
130 140 150 160 170 180
NEMVFALNGK PTFEIPYARI NNTNLTSKNE VGIEFNIQDE EYQPAGDELV EMRFYIPGVI
190 200 210 220 230 240
QTNVDENMTK KEESSNEVVP KKEDGAEGED VQMAVEEKSM AEAFYEELKE KADIGEVAGD
250 260 270 280 290 300
AIVSFQDVFF TTPRGRYDID IYKNSIRLRG KTYEYKLQHR QIQRIVSLPK ADDIHHLLVL
310 320 330 340 350 360
AIEPPLRQGQ TTYPFLVLQF QKDEETEVQL NLEDEDYEEN YKDKLKKQYD AKTHIVLSHV
370 380 390 400 410 420
LKGLTDRRVI VPGEYKSKYD QCAVSCSFKA NEGYLYPLDN AFFFLTKPTL YIPFSDVSMV
430 440 450 460 470 480
NISRAGQTST SSRTFDLEVV LRSNRGSTTF ANISKEEQQL LEQFLKSKNL RVKNEDREVQ
490 500 510 520 530 540
ERLQTALGSD SDEEDINMGS AGEDDESVDE DFQVSSDNDA DEVAEEFDSD AALSDAEGGS
550
DEERPSKKPK VE
References and
documentation are available.
Number of amino acids: 552
Molecular weight: 62993.80
Theoretical pI: 4.74
Total number of negatively charged residues (Asp + Glu): 101
Total number of positively charged residues (Arg + Lys): 68
Atomic composition:
Carbon C 2766
Hydrogen H 4304
Nitrogen N 758
Oxygen O 904
Sulfur S 11
Formula: C
2766H
4304N
758O
904S
11
Total number of atoms: 8743
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 51925
Abs 0.1% (=1 g/l) 0.824, assuming all pairs of Cys residues form cystines
Ext. coefficient 51800
Abs 0.1% (=1 g/l) 0.822, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 41.93
This classifies the protein as unstable.
Aliphatic index: 72.93
Grand average of hydropathicity (GRAVY): -0.743