ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSTDNSQKDE GVPLLSPYSS SPQLRKKKRN QKRRKDKFVG HLKSDSRRPT QLLHDNLQHN 

        70         80         90        100        110        120 
HGQITDFDQI DSWGMLHESD STSNDIIKSE DPSLKGAFID HRPSMSQPRE GPQSVSSTVQ 

       130        140        150        160        170        180 
PQPIMKFSTP SYKKPAGLRP SDQNRSLVSD LSPSELESWL KRRKSVHKSF VDENSPTDRR 

       190        200        210        220        230        240 
QSNANNDVVI DVDALMNHVN NNASTGVNDN SKRRKKKRGS DDSSNKNSKS TSSDSNDEED 

       250        260        270        280        290        300 
EYNSRPSSSL SSNNSSLDDV CLVLDDEGSE VPKAWPDCTV LEEFSKEETE RLRSQAIQDA 

       310        320        330        340        350        360 
EAFHFQYDED EEDGTSNEDG ILFSKPIVTN IDVPELGNRR VNETENLKNG RLRPKRIAPW 

       370        380        390        400        410        420 
HLIQRPMVLG SNSTKDSKSR IQSGLQDNLL VGRNIQYPPH IISNNPEHFR FTYFRVDLDS 

       430        440        450        460        470        480 
TVHSPTISGL LQPGQKFQDL FVASIYSQDN SAGHIKTHPN SPTPGIKAET VSQLQGLTAK 

       490        500        510        520        530        540 
NPSTLSSMSV ANIEDVPPFW LDVSNPTEEE MKILSKAFGI HPLTTEDIFL GEVREKVELF 

       550        560        570        580        590        600 
RDYYLICFRS FDIVAEKHVR RRRKEKQESA TLDHESISRR KSQAYGATMS NESNANNNNS 

       610        620        630        640        650        660 
TSNASRSKWL PSILRARRRS SANRTTNTSS SSYKRRVKSE KKKMEENEKF KRKSGDRHKP 

       670        680        690        700        710        720 
REGELEPLNV YIIVFRTGVL TFHFAPTPHP INVRRRARLL KDYLNVTSDW IAYALIDDIT 

       730        740        750        760        770        780 
DAFAPMIELI EDEVYEIEDA ILKMHQSDDS SDSDSSDSDS DSGASDEDAF PFDVYSKKTS 

       790        800        810        820        830        840 
YSSAKSSVSS RSMSTSEASF NANLIGWKRK GDMLRRIGEC RKRVMSILRL LGSKADVIKG 

       850        860        870        880        890        900 
FAKRYNEQWE ASPQSEIAMY LGDIQDHIVT MVSSLNHYEK LLSRSHSNYL AQINIDMTKV 

       910        920        930        940        950        960 
NNDMNDVLGK ITILGTIVLP MNVITGLWGM NVIVPGQYRD SLTWFIGIVL FMCMLACSAY 


MYTKRRFGF 

References and documentation are available.

Number of amino acids: 969

Molecular weight: 109736.56

Theoretical pI: 7.14

Amino acid composition: 
Ala (A)  43	  4.4%
Arg (R)  67	  6.9%
Asn (N)  62	  6.4%
Asp (D)  74	  7.6%
Cys (C)   6	  0.6%
Gln (Q)  34	  3.5%
Glu (E)  59	  6.1%
Gly (G)  42	  4.3%
His (H)  25	  2.6%
Ile (I)  55	  5.7%
Leu (L)  72	  7.4%
Lys (K)  65	  6.7%
Met (M)  24	  2.5%
Phe (F)  33	  3.4%
Pro (P)  47	  4.9%
Ser (S) 128	 13.2%
Thr (T)  46	  4.7%
Trp (W)  11	  1.1%
Tyr (Y)  24	  2.5%
Val (V)  52	  5.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 133
Total number of positively charged residues (Arg + Lys): 132

Atomic composition:

Carbon      C	      4761
Hydrogen    H	      7554
Nitrogen    N	      1392
Oxygen      O	      1530
Sulfur      S	        30

Formula: C4761H7554N1392O1530S30
Total number of atoms: 15267

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    96635
Abs 0.1% (=1 g/l)   0.881, assuming all pairs of Cys residues form cystines


Ext. coefficient    96260
Abs 0.1% (=1 g/l)   0.877, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 70.78
This classifies the protein as unstable.



Aliphatic index: 71.11

Grand average of hydropathicity (GRAVY): -0.758