ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSTDSTRYPI QIEKASNDPT LLLNHTCLRV KDPARTVKFY TEHFGMKLLS RKDFEEAKFS 

        70         80         90        100        110        120 
LYFLSFPKDD IPKNKNGEPD VFSAHGVLEL THNWGTEKNP DYKINNGNEE PHRGFGHICF 

       130        140        150        160        170        180 
SVSDINKTCE ELESQGVKFK KRLSEGRQKD IAFALGPDGY WIELITYSRE GQEYPKGSVG 

       190        200        210        220        230        240 
NKFNHTMIRI KNPTRSLEFY QNVLGMKLLR TSEHESAKFT LYFLGYGVPK TDSVFSCESV 

       250        260        270        280        290        300 
LELTHNWGTE NDPNFHYHNG NSEPQGYGHI CISCDDAGAL CKEIEVKYGD KIQWSPKFNQ 

       310        320 
GRMKNIAFLK DPDGYSIEVV PHGLIA 

References and documentation are available.

Number of amino acids: 326

Molecular weight: 37208.90

Theoretical pI: 6.36

Amino acid composition: 
Ala (A)  11	  3.4%
Arg (R)  12	  3.7%
Asn (N)  21	  6.4%
Asp (D)  18	  5.5%
Cys (C)   7	  2.1%
Gln (Q)   8	  2.5%
Glu (E)  26	  8.0%
Gly (G)  26	  8.0%
His (H)  13	  4.0%
Ile (I)  18	  5.5%
Leu (L)  25	  7.7%
Lys (K)  28	  8.6%
Met (M)   5	  1.5%
Phe (F)  19	  5.8%
Pro (P)  17	  5.2%
Ser (S)  23	  7.1%
Thr (T)  17	  5.2%
Trp (W)   4	  1.2%
Tyr (Y)  14	  4.3%
Val (V)  14	  4.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 44
Total number of positively charged residues (Arg + Lys): 40

Atomic composition:

Carbon      C	      1666
Hydrogen    H	      2537
Nitrogen    N	       449
Oxygen      O	       498
Sulfur      S	        12

Formula: C1666H2537N449O498S12
Total number of atoms: 5162

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    43235
Abs 0.1% (=1 g/l)   1.162, assuming all pairs of Cys residues form cystines


Ext. coefficient    42860
Abs 0.1% (=1 g/l)   1.152, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.67
This classifies the protein as unstable.



Aliphatic index: 67.27

Grand average of hydropathicity (GRAVY): -0.660