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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSTEDFYLRY YVGHKGKFGH EFLEFEFRPD GKLRYANNSN YKNDTMIRKE AFVHQSVMEE

70 80 90 100 110 120
LKRIIIDSEI MQEDDLPWPP PDRVGRQELE IVIGDEHISF TTSKTGSLVD VNRSKDPEGL

130 140
RCFYYLVQDL KCLVFSLIGL HFKIKPI


References and documentation are available.

Number of amino acids: 147 Molecular weight: 17310.79 Theoretical pI: 5.74
Amino acid composition: 
Ala (A) 2 1.4% Arg (R) 9 6.1% Asn (N) 5 3.4% Asp (D) 11 7.5% Cys (C) 2 1.4% Gln (Q) 4 2.7% Glu (E) 13 8.8% Gly (G) 9 6.1% His (H) 5 3.4% Ile (I) 11 7.5% Leu (L) 13 8.8% Lys (K) 11 7.5% Met (M) 4 2.7% Phe (F) 10 6.8% Pro (P) 7 4.8% Ser (S) 9 6.1% Thr (T) 5 3.4% Trp (W) 1 0.7% Tyr (Y) 7 4.8% Val (V) 9 6.1% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 24 Total number of positively charged residues (Arg + Lys): 20 Atomic composition: Carbon C 784 Hydrogen H 1205 Nitrogen N 205 Oxygen O 226 Sulfur S 6 Formula: C784H1205N205O226S6 Total number of atoms: 2426 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 16055 Abs 0.1% (=1 g/l) 0.927, assuming all pairs of Cys residues form cystines Ext. coefficient 15930 Abs 0.1% (=1 g/l) 0.920, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 46.22 This classifies the protein as unstable. Aliphatic index: 82.79 Grand average of hydropathicity (GRAVY): -0.473