ProtParam
User-provided sequence:
10 20 30 40 50 60
MSTEDFYLRY YVGHKGKFGH EFLEFEFRPD GKLRYANNSN YKNDTMIRKE AFVHQSVMEE
70 80 90 100 110 120
LKRIIIDSEI MQEDDLPWPP PDRVGRQELE IVIGDEHISF TTSKTGSLVD VNRSKDPEGL
130 140
RCFYYLVQDL KCLVFSLIGL HFKIKPI
References and
documentation are available.
Number of amino acids: 147
Molecular weight: 17310.79
Theoretical pI: 5.74
Total number of negatively charged residues (Asp + Glu): 24
Total number of positively charged residues (Arg + Lys): 20
Atomic composition:
Carbon C 784
Hydrogen H 1205
Nitrogen N 205
Oxygen O 226
Sulfur S 6
Formula: C
784H
1205N
205O
226S
6
Total number of atoms: 2426
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 16055
Abs 0.1% (=1 g/l) 0.927, assuming all pairs of Cys residues form cystines
Ext. coefficient 15930
Abs 0.1% (=1 g/l) 0.920, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 46.22
This classifies the protein as unstable.
Aliphatic index: 82.79
Grand average of hydropathicity (GRAVY): -0.473