ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSTEPVSASD KYQKISQLEH ILKRPDTYIG SVETQEQLQW IYDEETDCMI EKNVTIVPGL 

        70         80         90        100        110        120 
FKIFDEILVN AADNKVRDPS MKRIDVNIHA EEHTIEVKND GKGIPIEIHN KENIYIPEMI 

       130        140        150        160        170        180 
FGHLLTSSNY DDDEKKVTGG RNGYGAKLCN IFSTEFILET ADLNVGQKYV QKWENNMSIC 

       190        200        210        220        230        240 
HPPKITSYKK GPSYTKVTFK PDLTRFGMKE LDNDILGVMR RRVYDINGSV RDINVYLNGK 

       250        260        270        280        290        300 
SLKIRNFKNY VELYLKSLEK KRQLDNGEDG AAKSDIPTIL YERINNRWEV AFAVSDISFQ 

       310        320        330        340        350        360 
QISFVNSIAT TMGGTHVNYI TDQIVKKISE ILKKKKKKSV KSFQIKNNMF IFINCLIENP 

       370        380        390        400        410        420 
AFTSQTKEQL TTRVKDFGSR CEIPLEYINK IMKTDLATRM FEIADANEEN ALKKSDGTRK 

       430        440        450        460        470        480 
SRITNYPKLE DANKAGTKEG YKCTLVLTEG DSALSLAVAG LAVVGRDYYG CYPLRGKMLN 

       490        500        510        520        530        540 
VREASADQIL KNAEIQAIKK IMGLQHRKKY EDTKSLRYGH LMIMTDQDHD GSHIKGLIIN 

       550        560        570        580        590        600 
FLESSFPGLL DIQGFLLEFI TPIIKVSITK PTKNTIAFYN MPDYEKWREE ESHKFTWKQK 

       610        620        630        640        650        660 
YYKGLGTSLA QEVREYFSNL DRHLKIFHSL QGNDKDYIDL AFSKKKADDR KEWLRQYEPG 

       670        680        690        700        710        720 
TVLDPTLKEI PISDFINKEL ILFSLADNIR SIPNVLDGFK PGQRKVLYGC FKKNLKSELK 

       730        740        750        760        770        780 
VAQLAPYVSE CTAYHHGEQS LAQTIIGLAQ NFVGSNNIYL LLPNGAFGTR ATGGKDAAAA 

       790        800        810        820        830        840 
RYIYTELNKL TRKIFHPADD PLYKYIQEDE KTVEPEWYLP ILPMILVNGA EGIGTGWSTY 

       850        860        870        880        890        900 
IPPFNPLEII KNIRHLMNDE ELEQMHPWFR GWTGTIEEIE PLRYRMYGRI EQIGDNVLEI 

       910        920        930        940        950        960 
TELPARTWTS TIKEYLLLGL SGNDKIKPWI KDMEEQHDDN IKFIITLSPE EMAKTRKIGF 

       970        980        990       1000       1010       1020 
YERFKLISPI SLMNMVAFDP HGKIKKYNSV NEILSEFYYV RLEYYQKRKD HMSERLQWEV 

      1030       1040       1050       1060       1070       1080 
EKYSFQVKFI KMIIEKELTV TNKPRNAIIQ ELENLGFPRF NKEGKPYYGS PNDEIAEQIN 

      1090       1100       1110       1120       1130       1140 
DVKGATSDEE DEESSHEDTE NVINGPEELY GTYEYLLGMR IWSLTKERYQ KLLKQKQEKE 

      1150       1160       1170       1180       1190       1200 
TELENLLKLS AKDIWNTDLK AFEVGYQEFL QRDAEARGGN VPNKGSKTKG KGKRKLVDDE 

      1210       1220       1230       1240       1250       1260 
DYDPSKKNKK STARKGKKIK LEDKNFERIL LEQKLVTKSK APTKIKKEKT PSVSETKTEE 

      1270       1280       1290       1300       1310       1320 
EENAPSSTSS SSIFDIKKED KDEGELSKIS NKFKKISTIF DKMGSTSATS KENTPEQDDV 

      1330       1340       1350       1360       1370       1380 
ATKKNQTTAK KTAVKPKLAK KPVRKQQKVV ELSGESDLEI LDSYTDREDS NKDEDDAIPQ 

      1390       1400       1410       1420 
RSRRQRSSRA ASVPKKSYVE TLELSDDSFI EDDEEENQGS DVSFNEED 

References and documentation are available.

Number of amino acids: 1428

Molecular weight: 164214.73

Theoretical pI: 6.62

Amino acid composition: 
Ala (A)  65	  4.6%
Arg (R)  60	  4.2%
Asn (N)  80	  5.6%
Asp (D)  91	  6.4%
Cys (C)   9	  0.6%
Gln (Q)  50	  3.5%
Glu (E) 133	  9.3%
Gly (G)  78	  5.5%
His (H)  23	  1.6%
Ile (I) 116	  8.1%
Leu (L) 115	  8.1%
Lys (K) 159	 11.1%
Met (M)  28	  2.0%
Phe (F)  52	  3.6%
Pro (P)  56	  3.9%
Ser (S)  94	  6.6%
Thr (T)  83	  5.8%
Trp (W)  15	  1.1%
Tyr (Y)  60	  4.2%
Val (V)  61	  4.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 224
Total number of positively charged residues (Arg + Lys): 219

Atomic composition:

Carbon      C	      7327
Hydrogen    H	     11604
Nitrogen    N	      1958
Oxygen      O	      2244
Sulfur      S	        37

Formula: C7327H11604N1958O2244S37
Total number of atoms: 23170

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   172400
Abs 0.1% (=1 g/l)   1.050, assuming all pairs of Cys residues form cystines


Ext. coefficient   171900
Abs 0.1% (=1 g/l)   1.047, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.03
This classifies the protein as unstable.



Aliphatic index: 80.03

Grand average of hydropathicity (GRAVY): -0.697