ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSTFETLIKR GGNEAIKINP PTGADFHITS RGSDWFWTCF CCYLLFGLIL TFLMFRKPVN 

        70         80         90        100        110        120 
DRFFYLTGIA PNFFMCIAYF TMASNLGWIP VKAKYNHVQT STQKEHPGYR QIFYSRFVGW 

       130        140        150        160        170        180 
FLALPWPIIQ ICMLAGTPFW QMAFNVCITE FFTVCWLIAA CVHSTYKWGY YTIGLGAAIV 

       190        200        210        220        230        240 
VSISVMTTSY NLVKQRDNDI RLTFLVFFSI IMFLWIIAYP TCFGITDGGN VLQPDSAGIF 

       250        260        270        280        290        300 
YGIIDLILMC FIPTLLVPIA NHFGADKLGY HFGPSDAEAV MAPKAPVASP RPAATPNLSK 

       310        320 
DKKKKSKKSK KSKKSKKSEE 

References and documentation are available.

Number of amino acids: 320

Molecular weight: 36190.61

Theoretical pI: 9.32

Amino acid composition: 
Ala (A)  24	  7.5%
Arg (R)   9	  2.8%
Asn (N)  12	  3.8%
Asp (D)  11	  3.4%
Cys (C)  10	  3.1%
Gln (Q)   7	  2.2%
Glu (E)   7	  2.2%
Gly (G)  21	  6.6%
His (H)   6	  1.9%
Ile (I)  29	  9.1%
Leu (L)  24	  7.5%
Lys (K)  23	  7.2%
Met (M)  10	  3.1%
Phe (F)  28	  8.8%
Pro (P)  19	  5.9%
Ser (S)  20	  6.2%
Thr (T)  22	  6.9%
Trp (W)   9	  2.8%
Tyr (Y)  13	  4.1%
Val (V)  16	  5.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 18
Total number of positively charged residues (Arg + Lys): 32

Atomic composition:

Carbon      C	      1693
Hydrogen    H	      2556
Nitrogen    N	       410
Oxygen      O	       431
Sulfur      S	        20

Formula: C1693H2556N410O431S20
Total number of atoms: 5110

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    69495
Abs 0.1% (=1 g/l)   1.920, assuming all pairs of Cys residues form cystines


Ext. coefficient    68870
Abs 0.1% (=1 g/l)   1.903, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 30.71
This classifies the protein as stable.



Aliphatic index: 86.59

Grand average of hydropathicity (GRAVY): 0.251