ProtParam
User-provided sequence:
10 20 30 40 50 60
MSTFNAETAD NLEDIEKQFA VVAVEQAETY WKLLTSVPGS KLRLTKFDDE IYENFMERFP
70 80 90 100 110 120
EYKDVERVKK FTEEELKTKE AKERWRKFFT IFEKKIEDYN FGTLLRTDAS AEYGQFTTCF
130 140
VVRLQFYAFE IARNKHGLND WIVGQK
References and
documentation are available.
Number of amino acids: 146
Molecular weight: 17444.74
Theoretical pI: 5.24
Total number of negatively charged residues (Asp + Glu): 27
Total number of positively charged residues (Arg + Lys): 23
Atomic composition:
Carbon C 797
Hydrogen H 1207
Nitrogen N 201
Oxygen O 234
Sulfur S 3
Formula: C
797H
1207N
201O
234S
3
Total number of atoms: 2442
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 25440
Abs 0.1% (=1 g/l) 1.458, assuming all pairs of Cys residues form cystines
Ext. coefficient 25440
Abs 0.1% (=1 g/l) 1.458, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 42.62
This classifies the protein as unstable.
Aliphatic index: 67.47
Grand average of hydropathicity (GRAVY): -0.628