ProtParam
User-provided sequence:
10 20 30 40 50 60
MSTGDFLTKG IELVQKAIDL DTATQYEEAY TAYYNGLDYL MLALKYEKNP KSKDLIRAKF
70 80 90 100 110 120
TEYLNRAEQL KKHLESEEAN AAKKSPSAGS GSNGGNKKIS QEEGEDNGGE DNKKLRGALS
130 140 150 160 170 180
SAILSEKPNV KWEDVAGLEG AKEALKEAVI LPVKFPHLFK GNRKPTSGIL LYGPPGTGKS
190 200 210 220 230 240
YLAKAVATEA NSTFFSVSSS DLVSKWMGES EKLVKQLFAM ARENKPSIIF IDEVDALTGT
250 260 270 280 290 300
RGEGESEASR RIKTELLVQM NGVGNDSQGV LVLGATNIPW QLDSAIRRRF ERRIYIPLPD
310 320 330 340 350 360
LAARTTMFEI NVGDTPCVLT KEDYRTLGAM TEGYSGSDIA VVVKDALMQP IRKIQSATHF
370 380 390 400 410 420
KDVSTEDDET RKLTPCSPGD DGAIEMSWTD IEADELKEPD LTIKDFLKAI KSTRPTVNED
430
DLLKQEQFTR DFGQEGN
References and
documentation are available.
Number of amino acids: 437
Molecular weight: 48172.32
Theoretical pI: 5.12
Total number of negatively charged residues (Asp + Glu): 70
Total number of positively charged residues (Arg + Lys): 58
Atomic composition:
Carbon C 2119
Hydrogen H 3390
Nitrogen N 574
Oxygen O 682
Sulfur S 11
Formula: C
2119H
3390N
574O
682S
11
Total number of atoms: 6776
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 40005
Abs 0.1% (=1 g/l) 0.830, assuming all pairs of Cys residues form cystines
Ext. coefficient 39880
Abs 0.1% (=1 g/l) 0.828, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 34.68
This classifies the protein as stable.
Aliphatic index: 79.29
Grand average of hydropathicity (GRAVY): -0.537