ProtParam
User-provided sequence:
10 20 30 40 50 60
MSTGITYDED RKTQLIAQYE SVREVVNAEA KNVHVNENAS KILLLVVSKL KPASDIQILY
70 80 90 100 110 120
DHGVREFGEN YVQELIEKAK LLPDDIKWHF IGGLQTNKCK DLAKVPNLYS VETIDSLKKA
130 140 150 160 170 180
KKLNESRAKF QPDCNPILCN VQINTSHEDQ KSGLNNEAEI FEVIDFFLSE ECKYIKLNGL
190 200 210 220 230 240
MTIGSWNVSH EDSKENRDFA TLVEWKKKID AKFGTSLKLS MGMSADFREA IRQGTAEVRI
250
GTDIFGARPP KNEARII
References and
documentation are available.
Number of amino acids: 257
Molecular weight: 29123.22
Theoretical pI: 6.03
Total number of negatively charged residues (Asp + Glu): 39
Total number of positively charged residues (Arg + Lys): 36
Atomic composition:
Carbon C 1292
Hydrogen H 2066
Nitrogen N 352
Oxygen O 396
Sulfur S 8
Formula: C
1292H
2066N
352O
396S
8
Total number of atoms: 4114
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 25690
Abs 0.1% (=1 g/l) 0.882, assuming all pairs of Cys residues form cystines
Ext. coefficient 25440
Abs 0.1% (=1 g/l) 0.874, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 26.77
This classifies the protein as stable.
Aliphatic index: 89.92
Grand average of hydropathicity (GRAVY): -0.454