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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSTIGAVDIL NQKTITSEVA ASVTSKYLQS TFSKGNTSHI EDKRFIHVSS RSHSRFTSTP

70 80 90 100 110 120
ITPNEILSLK FHVSGSSMAY SRMDGSLTVW FIKDASFDKS VEVYIPDCCG SDKLATDLSW

130 140 150 160 170 180
NPTSLNQIAV VSNSSEISLL LINEKSLTAS KLRTLSLGSK TKVNTCLYDP LGNWLLAATK

190 200 210 220 230 240
SEKIYLFDVK KDHSSVCSLN ISDISQEDND VVYSLAWSNG GSHIFIGFKS GYLAILKAKH

250 260 270 280 290 300
GILEVCTKIK AHTGPITEIK MDPWGRNFIT GSIDGNCYVW NMKSLCCELI INDLNSAVTT

310 320 330 340 350 360
LDVCHLGKIL GICTEDEMVY FYDLNSGNLL HSKSLANYKT DPVLKFYPDK SWYIMSGKND

370 380 390 400 410 420
TLSNHFVKNE KNLITYWKDM FDNTMIEKRR KNNGGGNNHN KRTSKNTDRI GKDRPSRFNS


KK


References and documentation are available.

Number of amino acids: 422 Molecular weight: 47166.70 Theoretical pI: 8.88
Amino acid composition: 
Ala (A) 16 3.8% Arg (R) 12 2.8% Asn (N) 32 7.6% Asp (D) 27 6.4% Cys (C) 10 2.4% Gln (Q) 4 0.9% Glu (E) 15 3.6% Gly (G) 24 5.7% His (H) 12 2.8% Ile (I) 32 7.6% Leu (L) 38 9.0% Lys (K) 39 9.2% Met (M) 9 2.1% Phe (F) 15 3.6% Pro (P) 10 2.4% Ser (S) 53 12.6% Thr (T) 30 7.1% Trp (W) 8 1.9% Tyr (Y) 14 3.3% Val (V) 22 5.2% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 42 Total number of positively charged residues (Arg + Lys): 51 Atomic composition: Carbon C 2088 Hydrogen H 3299 Nitrogen N 565 Oxygen O 640 Sulfur S 19 Formula: C2088H3299N565O640S19 Total number of atoms: 6611 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 65485 Abs 0.1% (=1 g/l) 1.388, assuming all pairs of Cys residues form cystines Ext. coefficient 64860 Abs 0.1% (=1 g/l) 1.375, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 35.71 This classifies the protein as stable. Aliphatic index: 83.60 Grand average of hydropathicity (GRAVY): -0.327