ProtParam
User-provided sequence:
10 20 30 40 50 60
MSTIGAVDIL NQKTITSEVA ASVTSKYLQS TFSKGNTSHI EDKRFIHVSS RSHSRFTSTP
70 80 90 100 110 120
ITPNEILSLK FHVSGSSMAY SRMDGSLTVW FIKDASFDKS VEVYIPDCCG SDKLATDLSW
130 140 150 160 170 180
NPTSLNQIAV VSNSSEISLL LINEKSLTAS KLRTLSLGSK TKVNTCLYDP LGNWLLAATK
190 200 210 220 230 240
SEKIYLFDVK KDHSSVCSLN ISDISQEDND VVYSLAWSNG GSHIFIGFKS GYLAILKAKH
250 260 270 280 290 300
GILEVCTKIK AHTGPITEIK MDPWGRNFIT GSIDGNCYVW NMKSLCCELI INDLNSAVTT
310 320 330 340 350 360
LDVCHLGKIL GICTEDEMVY FYDLNSGNLL HSKSLANYKT DPVLKFYPDK SWYIMSGKND
370 380 390 400 410 420
TLSNHFVKNE KNLITYWKDM FDNTMIEKRR KNNGGGNNHN KRTSKNTDRI GKDRPSRFNS
KK
References and
documentation are available.
Number of amino acids: 422
Molecular weight: 47166.70
Theoretical pI: 8.88
Total number of negatively charged residues (Asp + Glu): 42
Total number of positively charged residues (Arg + Lys): 51
Atomic composition:
Carbon C 2088
Hydrogen H 3299
Nitrogen N 565
Oxygen O 640
Sulfur S 19
Formula: C
2088H
3299N
565O
640S
19
Total number of atoms: 6611
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 65485
Abs 0.1% (=1 g/l) 1.388, assuming all pairs of Cys residues form cystines
Ext. coefficient 64860
Abs 0.1% (=1 g/l) 1.375, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 35.71
This classifies the protein as stable.
Aliphatic index: 83.60
Grand average of hydropathicity (GRAVY): -0.327