ProtParam
User-provided sequence:
10 20 30 40 50 60
MSTIPSEIIN WTILNEIISM DDDDSDFSKG LIIQFIDQAQ TTFAQMQRQL DGEKNLTELD
70 80 90 100 110 120
NLGHFLKGSS AALGLQRIAW VCERIQNLGR KMEHFFPNKT ELVNTLSDKS IINGINIDED
130 140 150 160
DEEIKIQVDD KDENSIYLIL IAKALNQSRL EFKLARIELS KYYNTNL
References and
documentation are available.
Number of amino acids: 167
Molecular weight: 19168.70
Theoretical pI: 4.58
Total number of negatively charged residues (Asp + Glu): 28
Total number of positively charged residues (Arg + Lys): 17
Atomic composition:
Carbon C 847
Hydrogen H 1353
Nitrogen N 225
Oxygen O 270
Sulfur S 5
Formula: C
847H
1353N
225O
270S
5
Total number of atoms: 2700
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 15470
Abs 0.1% (=1 g/l) 0.807, assuming all pairs of Cys residues form cystines
Ext. coefficient 15470
Abs 0.1% (=1 g/l) 0.807, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 30.72
This classifies the protein as stable.
Aliphatic index: 103.41
Grand average of hydropathicity (GRAVY): -0.349