ProtParam
User-provided sequence:
10 20 30 40 50 60
MSTKIKGELF PSRSLVSKKW TFLLCFGSFC FGILFTDRMW IIPESKDMPR PSVSTEAERL
70 80 90 100 110 120
KLISEGCDPK TLYQKEVNRD PQALFGEVSK THNAIQTLDK TISSLEMELA AARSAQESLV
130 140 150 160 170 180
NGAPISNDME KKQLPGKRRY LMVVGINTAF SSRKRRDSVR TTWMPSGEKR KKLEEEKGII
190 200 210 220 230 240
IRFVIGHSAT AGGILDRSIE AEDKKHGDFL RLDHVEGYLE LSGKTKTYFS TAVSKWDAEF
250 260 270 280 290 300
YVKVDDDVHV NIATLGETLV RHRKKHRVYL GCMKSGPVLS QKGVRYHEPE YWKFGENGNK
310 320 330 340 350 360
YFRHATGQLY AISRDLASYI SLNQHVLHKY ANEDVTLGAW FIGLDVTHID DRRLCCGTPP
370 380 390 400
DCEWKAQAGN ICVASFDWTC SGICRSADRI KEVHKRCGEP ENAIWKARF
References and
documentation are available.
Number of amino acids: 409
Molecular weight: 46483.21
Theoretical pI: 9.01
Total number of negatively charged residues (Asp + Glu): 52
Total number of positively charged residues (Arg + Lys): 63
Atomic composition:
Carbon C 2065
Hydrogen H 3250
Nitrogen N 584
Oxygen O 601
Sulfur S 19
Formula: C
2065H
3250N
584O
601S
19
Total number of atoms: 6519
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 68005
Abs 0.1% (=1 g/l) 1.463, assuming all pairs of Cys residues form cystines
Ext. coefficient 67380
Abs 0.1% (=1 g/l) 1.450, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 41.58
This classifies the protein as unstable.
Aliphatic index: 76.77
Grand average of hydropathicity (GRAVY): -0.467