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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSTKIKGELF PSRSLVSKKW TFLLCFGSFC FGILFTDRMW IIPESKDMPR PSVSTEAERL

70 80 90 100 110 120
KLISEGCDPK TLYQKEVNRD PQALFGEVSK THNAIQTLDK TISSLEMELA AARSAQESLV

130 140 150 160 170 180
NGAPISNDME KKQLPGKRRY LMVVGINTAF SSRKRRDSVR TTWMPSGEKR KKLEEEKGII

190 200 210 220 230 240
IRFVIGHSAT AGGILDRSIE AEDKKHGDFL RLDHVEGYLE LSGKTKTYFS TAVSKWDAEF

250 260 270 280 290 300
YVKVDDDVHV NIATLGETLV RHRKKHRVYL GCMKSGPVLS QKGVRYHEPE YWKFGENGNK

310 320 330 340 350 360
YFRHATGQLY AISRDLASYI SLNQHVLHKY ANEDVTLGAW FIGLDVTHID DRRLCCGTPP

370 380 390 400
DCEWKAQAGN ICVASFDWTC SGICRSADRI KEVHKRCGEP ENAIWKARF


References and documentation are available.

Number of amino acids: 409 Molecular weight: 46483.21 Theoretical pI: 9.01
Amino acid composition: 
Ala (A) 26 6.4% Arg (R) 28 6.8% Asn (N) 12 2.9% Asp (D) 23 5.6% Cys (C) 11 2.7% Gln (Q) 9 2.2% Glu (E) 29 7.1% Gly (G) 29 7.1% His (H) 13 3.2% Ile (I) 24 5.9% Leu (L) 32 7.8% Lys (K) 35 8.6% Met (M) 8 2.0% Phe (F) 17 4.2% Pro (P) 14 3.4% Ser (S) 32 7.8% Thr (T) 22 5.4% Trp (W) 9 2.2% Tyr (Y) 12 2.9% Val (V) 24 5.9% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 52 Total number of positively charged residues (Arg + Lys): 63 Atomic composition: Carbon C 2065 Hydrogen H 3250 Nitrogen N 584 Oxygen O 601 Sulfur S 19 Formula: C2065H3250N584O601S19 Total number of atoms: 6519 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 68005 Abs 0.1% (=1 g/l) 1.463, assuming all pairs of Cys residues form cystines Ext. coefficient 67380 Abs 0.1% (=1 g/l) 1.450, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 41.58 This classifies the protein as unstable. Aliphatic index: 76.77 Grand average of hydropathicity (GRAVY): -0.467