ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSTLKQRKED FVTGLNGGSI TEINAVTSIA LVTYISWNLL KNSNLMPPGI SSVQYIIDFA 

        70         80         90        100        110        120 
LNWVALLLSI TIYASEPYLL NTLILLPCLL AFIYGKFTSS SKPSNPIYNK KKMITQRFQL 

       130        140        150        160        170        180 
EKKPYITAYR GGMLILTAIA ILAVDFPIFP RRFAKVETWG TSLMDLGVGS FVFSNGIVSS 

       190        200        210        220        230        240 
RALLKNLSLK SKPSFLKNAF NALKSGGTLL FLGLLRLFFV KNLEYQEHVT EYGVHWNFFI 

       250        260        270        280        290        300 
TLSLLPLVLT FIDPVTRMVP RCSIAIFISC IYEWLLLKDD RTLNFLILAD RNCFFSANRE 

       310        320        330        340        350        360 
GIFSFLGYCS IFLWGQNTGF YLLGNKPTLN NLYKPSTQDV VAASKKSSTW DYWTSVTPLS 

       370        380        390        400        410        420 
GLCIWSTIFL VISQLVFQYH PYSVSRRFAN LPYTLWVITY NLLFLTGYCL TDKIFGNSSE 

       430        440        450        460        470        480 
YYKVAECLES INSNGLFLFL LANVSTGLVN MSMVTIDSSP LKSFLVLLAY CSFIAVISVF 

       490 
LYRKRIFIKL 

References and documentation are available.

Number of amino acids: 490

Molecular weight: 55466.32

Theoretical pI: 9.41

Amino acid composition: 
Ala (A)  24	  4.9%
Arg (R)  16	  3.3%
Asn (N)  29	  5.9%
Asp (D)  12	  2.4%
Cys (C)   9	  1.8%
Gln (Q)   9	  1.8%
Glu (E)  13	  2.7%
Gly (G)  25	  5.1%
His (H)   3	  0.6%
Ile (I)  39	  8.0%
Leu (L)  75	 15.3%
Lys (K)  27	  5.5%
Met (M)   8	  1.6%
Phe (F)  37	  7.6%
Pro (P)  19	  3.9%
Ser (S)  49	 10.0%
Thr (T)  32	  6.5%
Trp (W)  10	  2.0%
Tyr (Y)  24	  4.9%
Val (V)  30	  6.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 25
Total number of positively charged residues (Arg + Lys): 43

Atomic composition:

Carbon      C	      2602
Hydrogen    H	      4023
Nitrogen    N	       619
Oxygen      O	       684
Sulfur      S	        17

Formula: C2602H4023N619O684S17
Total number of atoms: 7945

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    91260
Abs 0.1% (=1 g/l)   1.645, assuming all pairs of Cys residues form cystines


Ext. coefficient    90760
Abs 0.1% (=1 g/l)   1.636, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 24.81
This classifies the protein as stable.



Aliphatic index: 113.39

Grand average of hydropathicity (GRAVY): 0.452