ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSTLLERTKS VQELKKRAAG KTSANPAEVA KAKKVLRRLY SWDEIPEWQR DNDFILHGYV 

        70         80         90        100        110        120 
KETSSFIETF KSLFYLHNES VNIYSHLIPA LGFFTVLLLD KSTIKVFATT TWLDHMVIDL 

       130        140        150        160        170        180 
FYSGAFACLI LSSSFHCLKS HSLRIATLGN KLDYLGICIL IVTSMVSILY YGYFEKFSLF 

       190        200        210        220        230        240 
CLFALITVSF GIACSIVSLK DKFRKREWRP YRAGLFVCFG LSSIIPIFSG LYCYSFSEIW 

       250        260        270        280        290        300 
TQIQLFWVLL GGVLYIIGAV LYGMRFPEKI CPGKFDIWGH SHQLFHFLVV IAALCHLRGL 

       310 
LNSYELVHIK MENGIVS 

References and documentation are available.

Number of amino acids: 317

Molecular weight: 36278.78

Theoretical pI: 9.03

Amino acid composition: 
Ala (A)  17	  5.4%
Arg (R)  12	  3.8%
Asn (N)   7	  2.2%
Asp (D)   9	  2.8%
Cys (C)   9	  2.8%
Gln (Q)   5	  1.6%
Glu (E)  14	  4.4%
Gly (G)  19	  6.0%
His (H)  11	  3.5%
Ile (I)  28	  8.8%
Leu (L)  43	 13.6%
Lys (K)  20	  6.3%
Met (M)   5	  1.6%
Phe (F)  25	  7.9%
Pro (P)   7	  2.2%
Ser (S)  30	  9.5%
Thr (T)  14	  4.4%
Trp (W)   7	  2.2%
Tyr (Y)  15	  4.7%
Val (V)  20	  6.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 23
Total number of positively charged residues (Arg + Lys): 32

Atomic composition:

Carbon      C	      1702
Hydrogen    H	      2608
Nitrogen    N	       414
Oxygen      O	       435
Sulfur      S	        14

Formula: C1702H2608N414O435S14
Total number of atoms: 5173

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    61350
Abs 0.1% (=1 g/l)   1.691, assuming all pairs of Cys residues form cystines


Ext. coefficient    60850
Abs 0.1% (=1 g/l)   1.677, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 31.85
This classifies the protein as stable.



Aliphatic index: 111.01

Grand average of hydropathicity (GRAVY): 0.435