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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSTLSYVSPD ALKGWLMESP NEISIIDVRD YDYEGERIPG SVRIPSDTFL ASVDQHVDDL

70 80 90 100 110 120
MKKRSLIVHC TYSQVRGPKA ARVLSEILRN RITESKEKLS LSQKEKLFQN LPTVYILHGG

130
FSAWKRRYGG QQGLIEYD


References and documentation are available.

Number of amino acids: 138 Molecular weight: 15778.00 Theoretical pI: 7.84
Amino acid composition: 
Ala (A) 5 3.6% Arg (R) 10 7.2% Asn (N) 3 2.2% Asp (D) 9 6.5% Cys (C) 1 0.7% Gln (Q) 6 4.3% Glu (E) 9 6.5% Gly (G) 9 6.5% His (H) 3 2.2% Ile (I) 10 7.2% Leu (L) 14 10.1% Lys (K) 9 6.5% Met (M) 3 2.2% Phe (F) 3 2.2% Pro (P) 6 4.3% Ser (S) 15 10.9% Thr (T) 5 3.6% Trp (W) 2 1.4% Tyr (Y) 7 5.1% Val (V) 9 6.5% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 18 Total number of positively charged residues (Arg + Lys): 19 Atomic composition: Carbon C 702 Hydrogen H 1116 Nitrogen N 194 Oxygen O 211 Sulfur S 4 Formula: C702H1116N194O211S4 Total number of atoms: 2227 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 21430 Abs 0.1% (=1 g/l) 1.358, assuming all pairs of Cys residues form cystines Ext. coefficient 21430 Abs 0.1% (=1 g/l) 1.358, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 53.40 This classifies the protein as unstable. Aliphatic index: 90.36 Grand average of hydropathicity (GRAVY): -0.451