ProtParam
User-provided sequence:
10 20 30 40 50 60
MSTMSTPAAE QRKLVEQLMG RDFSFRHNRY SHQKRDLGLH DPKICKSYLV GECPYDLFQG
70 80 90 100 110 120
TKQSLGKCPQ MHLTKHKIQY EREVKQGKTF PEFEREYLAI LSRFVNECNG QISVALQNLK
130 140 150 160 170 180
HTAEERMKIQ QVTEELDVLD VRIGLMGQEI DSLIRADEVS MGMLQSVKLQ ELISKRKEVA
190 200 210 220 230 240
KRVRNITENV GQSAQQKLQV CEVCGAYLSR LDTDRRLADH FLGKIHLGYV KMREDYDRLM
250 260
KNNRTTNASK TATTLPGRRF V
References and
documentation are available.
Number of amino acids: 261
Molecular weight: 30194.76
Theoretical pI: 9.31
Total number of negatively charged residues (Asp + Glu): 33
Total number of positively charged residues (Arg + Lys): 43
Atomic composition:
Carbon C 1311
Hydrogen H 2140
Nitrogen N 392
Oxygen O 393
Sulfur S 16
Formula: C
1311H
2140N
392O
393S
16
Total number of atoms: 4252
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 12295
Abs 0.1% (=1 g/l) 0.407, assuming all pairs of Cys residues form cystines
Ext. coefficient 11920
Abs 0.1% (=1 g/l) 0.395, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 53.71
This classifies the protein as unstable.
Aliphatic index: 80.27
Grand average of hydropathicity (GRAVY): -0.637