ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSTNTSKTLE LFSKKTVQEH NTANDCWVTY QNRKIYDVTR FLSEHPGGDE SILDYAGKDI 

        70         80         90        100        110        120 
TEIMKDSDVH EHSDSAYEIL EDEYLIGYLA TDEEAARLLT NKNHKVEVQL SADGTEFDST 

       130        140        150        160        170        180 
TFVKELPAEE KLSIATDYSN DYKKHKFLDL NRPLLMQILR SDFKKDFYVD QIHRPRHYGK 

       190        200        210        220        230        240 
GSAPLFGNFL EPLTKTAWWV VPVAWLPVVV YHMGVALKNM NQLFACFLFC VGVFVWTLIE 

       250        260        270        280        290        300 
YGLHRFLFHF DDWLPESNIA FATHFLLHGC HHYLPMDKYR LVMPPTLFVI LCAPFYKLVF 

       310        320        330        340        350        360 
ALLPLYWAYA GFAGGLFGYV CYDECHFFLH HSKLPPFMRK LKKYHLEHHY KNYQLGFGVT 

       370        380 
SWFWDEVFGT YLGPDAPLSK MKYE 

References and documentation are available.

Number of amino acids: 384

Molecular weight: 44881.48

Theoretical pI: 6.07

Amino acid composition: 
Ala (A)  22	  5.7%
Arg (R)  10	  2.6%
Asn (N)  13	  3.4%
Asp (D)  24	  6.2%
Cys (C)   7	  1.8%
Gln (Q)   7	  1.8%
Glu (E)  23	  6.0%
Gly (G)  20	  5.2%
His (H)  21	  5.5%
Ile (I)  12	  3.1%
Leu (L)  46	 12.0%
Lys (K)  27	  7.0%
Met (M)   9	  2.3%
Phe (F)  29	  7.6%
Pro (P)  18	  4.7%
Ser (S)  18	  4.7%
Thr (T)  21	  5.5%
Trp (W)   9	  2.3%
Tyr (Y)  24	  6.2%
Val (V)  24	  6.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 47
Total number of positively charged residues (Arg + Lys): 37

Atomic composition:

Carbon      C	      2090
Hydrogen    H	      3078
Nitrogen    N	       512
Oxygen      O	       562
Sulfur      S	        16

Formula: C2090H3078N512O562S16
Total number of atoms: 6258

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    85635
Abs 0.1% (=1 g/l)   1.908, assuming all pairs of Cys residues form cystines


Ext. coefficient    85260
Abs 0.1% (=1 g/l)   1.900, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.62
This classifies the protein as unstable.



Aliphatic index: 82.76

Grand average of hydropathicity (GRAVY): -0.188