ProtParam
User-provided sequence:
10 20 30 40 50 60
MSTPANYTRV PLCEPEELPD DIQKENEYGT LDSPGHLYQV KSRHGKPLPE PVVDTPPYYI
70 80 90 100 110 120
SLLTYLNYLI LIILGHVHDF LGMTFQKNKH LDLLEHDGLA PWFSNFESFY VRRIKMRIDD
130 140 150 160 170 180
CFSRPTTGVP GRFIRCIDRI SHNINEYFTY SGAVYPCMNL SSYNYLGFAQ SKGQCTDAAL
190 200 210 220 230 240
ESVDKYSIQS GGPRAQIGTT DLHIKAEKLV ARFIGKEDAL VFSMGYGTNA NLFNAFLDKK
250 260 270 280 290 300
CLVISDELNH TSIRTGVRLS GAAVRTFKHG DMVGLEKLIR EQIVLGQPKT NRPWKKILIC
310 320 330 340 350 360
AEGLFSMEGT LCNLPKLVEL KKKYKCYLFI DEAHSIGAMG PTGRGVCEIF GVDPKDVDIL
370 380 390 400 410 420
MGTFTKSFGA AGGYIAADQW IIDRLRLDLT TVSYSESMPA PVLAQTISSL QTISGEICPG
430 440 450 460 470 480
QGTERLQRIA FNSRYLRLAL QRLGFIVYGV ADSPVIPLLL YCPSKMPAFS RMMLQRRIAV
490 500 510 520 530 540
VVVAYPATPL IESRVRFCMS ASLTKEDIDY LLRHVSEVGD KLNLKSNSGK SSYDGKRQRW
550 560
DIEEVIRRTP EDCKDDKYFV N
References and
documentation are available.
Number of amino acids: 561
Molecular weight: 63110.82
Theoretical pI: 8.38
Total number of negatively charged residues (Asp + Glu): 62
Total number of positively charged residues (Arg + Lys): 67
Atomic composition:
Carbon C 2831
Hydrogen H 4458
Nitrogen N 760
Oxygen O 817
Sulfur S 28
Formula: C
2831H
4458N
760O
817S
28
Total number of atoms: 8894
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 61615
Abs 0.1% (=1 g/l) 0.976, assuming all pairs of Cys residues form cystines
Ext. coefficient 60740
Abs 0.1% (=1 g/l) 0.962, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 42.49
This classifies the protein as unstable.
Aliphatic index: 90.53
Grand average of hydropathicity (GRAVY): -0.167