ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSTQQALAQA NEHALSQAND LIKVGSKLRA LSVLRDLLSD RSQWHSSLEQ IMSLYVSLCA 

        70         80         90        100        110        120 
EQLDYQSLRD GIHHFKVSIM SQKEFSIVPL ENIFKEIITP IEQKVDELKE KIEKENQENP 

       130        140        150        160        170        180 
LVEQNEISLI DPQQTLLFSY MKYLFEAYKA MIEVLTRQNT KFEHTKFDHS KFDSTLLKIS 

       190        200        210        220        230        240 
TQALNYCSKY QRKPDFMVLT ELFRSSIEQL FKVPSLDTVN THIEIRFHQL TVAISLGLYL 

       250        260        270        280        290        300 
IAYKSIEDIN IMLFSLLVKP KPVVLATYYQ KLAQVYWITN AHLLHAYALY KHYVYNKNYN 

       310        320        330        340        350        360 
MNFTQADSQL YSSVLLVAAL SSPIQEVNQN QSLLQFDSQS QRAMGLASLL SLQSIPKRET 

       370        380        390        400        410        420 
FLVDVRKVTN EVYPELADLA SIFEKKTSPL MFAKLLEPKI KFIEGHAQLS QYLKPFLRVV 

       430        440        450        460        470        480 
FTKIALQVSK VYEVIKIEEF IKLVPFYTKT QIELYLLESI KRKLIGARID HKNGVIRFGH 

       490        500        510        520        530        540 
YDFDSAKISD QLSNLATGVG KALNMIEPEK KQQQHDKLKK EVYVKIINSL QDEHRRILAR 

       550        560        570        580        590        600 
KEIIEKKKIY MEQQDRIKKQ KEHEELQKKI QEKVARDQQR LKEDMERREK EQAEEESQQN 

       610        620        630        640        650        660 
QLDQTINAID KAKVEMKAKI AKIAKQLYIL ERAYREEELP VVESLQKTKA VEDKQYFEST 

       670        680        690        700        710        720 
QAEFLKLHRE VHDRNVTEKA RLNRIVPEYQ KFTQAVIEER KKQLPALQKE QEKRFQEFLI 

       730        740        750        760        770        780 
QQEQDVQERK AKREKARIAA AQEKARKEEQ ERERLEQEHL EQERLEEERK NAPYVPPSSR 

       790        800        810        820        830        840 
RTFRDDDDER EESGRWGGRR GGDDFGRSKA DEGDRWGRRE DAPPPRRDEG GDRWGRREDA 

       850        860        870        880        890        900 
PPPRRDEGGD RWGKREDAPP PRRDEGGWGR RDDAPPPRRD EGGDRWGRRD DAPPRRDDAP 

       910        920        930        940        950        960 
PPRRDDAPPP RRDEGGDRWG RRDDAPPRRD GGGSGGFGGR RDDAPPRRDE GGDRWGRRED 

       970        980        990       1000       1010       1020 
APPPRRDEGG DRWGRRDDAP PRRDGGGGSG FGRNQGAQGD QNDSWRSDNK KEENKKDADG 

      1030 
WQTVGAKKRY 

References and documentation are available.

Number of amino acids: 1030

Molecular weight: 120076.47

Theoretical pI: 8.85

Amino acid composition: 
Ala (A)  67	  6.5%
Arg (R)  96	  9.3%
Asn (N)  31	  3.0%
Asp (D)  76	  7.4%
Cys (C)   2	  0.2%
Gln (Q)  73	  7.1%
Glu (E) 100	  9.7%
Gly (G)  56	  5.4%
His (H)  20	  1.9%
Ile (I)  56	  5.4%
Leu (L)  87	  8.4%
Lys (K)  90	  8.7%
Met (M)  14	  1.4%
Phe (F)  34	  3.3%
Pro (P)  51	  5.0%
Ser (S)  57	  5.5%
Thr (T)  29	  2.8%
Trp (W)  13	  1.3%
Tyr (Y)  32	  3.1%
Val (V)  46	  4.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 176
Total number of positively charged residues (Arg + Lys): 186

Atomic composition:

Carbon      C	      5285
Hydrogen    H	      8419
Nitrogen    N	      1565
Oxygen      O	      1605
Sulfur      S	        16

Formula: C5285H8419N1565O1605S16
Total number of atoms: 16890

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   119305
Abs 0.1% (=1 g/l)   0.994, assuming all pairs of Cys residues form cystines


Ext. coefficient   119180
Abs 0.1% (=1 g/l)   0.993, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 67.05
This classifies the protein as unstable.



Aliphatic index: 73.60

Grand average of hydropathicity (GRAVY): -0.997