ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSTQQQSYTI WSPQDTVKDV AESLGLENIN DDVLKALAMD VEYRILEIIE QAVKFKRHSK 

        70         80         90        100        110        120 
RDVLTTDDVS KALRVLNVEP LYGYYDGSEV NKAVSFSKVN TSGGQSVYYL DEEEVDFDRL 

       130        140        150        160        170        180 
INEPLPQVPR LPTFTTHWLA VEGVQPAIIQ NPNLNDIRVS QPPFIRGAIV TALNDNSLQT 

       190        200        210        220        230        240 
PVTSTTASAS VTDTGASQHL SNVKPGQNTE VKPLVKHVLS KELQIYFNKV ISTLTAKSQA 

       250        260        270        280        290        300 
DEAAQHMKQA ALTSLRTDSG LHQLVPYFIQ FIAEQITQNL SDLQLLTTIL EMIYSLLSNT 

       310        320        330        340        350        360 
SIFLDPYIHS LMPSILTLLL AKKLGGSPKD DSPQEIHEFL ERTNALRDFA ASLLDYVLKK 

       370        380        390        400        410        420 
FPQAYKSLKP RVTRTLLKTF LDINRVFGTY YGCLKGVSVL EGESIRFFLG NLNNWARLVF 

       430        440        450        460        470        480 
NESGITLDNI EEHLNDDSNP TRTKFTKEET QILVDTVISA LLVLKKDLPD LYEGKGEKVT 

       490        500        510 
DEDKEKLLER CGVTIGFHIL KRDDAKELIS AIFFGE 

References and documentation are available.

Number of amino acids: 516

Molecular weight: 57901.97

Theoretical pI: 5.26

Amino acid composition: 
Ala (A)  29	  5.6%
Arg (R)  19	  3.7%
Asn (N)  25	  4.8%
Asp (D)  34	  6.6%
Cys (C)   2	  0.4%
Gln (Q)  25	  4.8%
Glu (E)  34	  6.6%
Gly (G)  23	  4.5%
His (H)  10	  1.9%
Ile (I)  32	  6.2%
Leu (L)  66	 12.8%
Lys (K)  34	  6.6%
Met (M)   5	  1.0%
Phe (F)  21	  4.1%
Pro (P)  22	  4.3%
Ser (S)  39	  7.6%
Thr (T)  39	  7.6%
Trp (W)   3	  0.6%
Tyr (Y)  16	  3.1%
Val (V)  38	  7.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 68
Total number of positively charged residues (Arg + Lys): 53

Atomic composition:

Carbon      C	      2600
Hydrogen    H	      4140
Nitrogen    N	       680
Oxygen      O	       797
Sulfur      S	         7

Formula: C2600H4140N680O797S7
Total number of atoms: 8224

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    40465
Abs 0.1% (=1 g/l)   0.699, assuming all pairs of Cys residues form cystines


Ext. coefficient    40340
Abs 0.1% (=1 g/l)   0.697, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 36.79
This classifies the protein as stable.



Aliphatic index: 101.05

Grand average of hydropathicity (GRAVY): -0.212