ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSTQSGIVAE QALLHSLNEN LSADGIVIII AKISPDSTSV HQTQVARSFE ELVQLASQER 

        70         80         90        100        110        120 
EPLYIFYKPE GLDKYFFVSF IPDGSPVRSR MLYASTKNTL ARQVGSNSLS TEQPLITDAQ 

       130        140        150        160        170        180 
DLVDLKNFDS ARPAGQNKPL THDEEMQIEI NKQQALLRKN TSVKLVSQDS ASPLSLTFRV 

       190        200        210        220        230        240 
NSEKPINEIL DSEGKNLIIF QIDPSNETIQ IVQSDTCPSV DELYIDLPGP SYTIFRQGDS 

       250        260        270        280        290        300 
SFFIYSCPSG SKVKDRMIYA SNKNGFINYL KNDQKIAFSK VVEIGDFVEL DKSLLMATNK 

       310        320        330 
EDSLDHGSNP DLPNKSNLKF NKPKGPLRKR RT 

References and documentation are available.

Number of amino acids: 332

Molecular weight: 37070.87

Theoretical pI: 5.55

Amino acid composition: 
Ala (A)  16	  4.8%
Arg (R)  13	  3.9%
Asn (N)  21	  6.3%
Asp (D)  23	  6.9%
Cys (C)   2	  0.6%
Gln (Q)  19	  5.7%
Glu (E)  19	  5.7%
Gly (G)  14	  4.2%
His (H)   4	  1.2%
Ile (I)  25	  7.5%
Leu (L)  32	  9.6%
Lys (K)  24	  7.2%
Met (M)   5	  1.5%
Phe (F)  15	  4.5%
Pro (P)  19	  5.7%
Ser (S)  39	 11.7%
Thr (T)  15	  4.5%
Trp (W)   0	  0.0%
Tyr (Y)   9	  2.7%
Val (V)  18	  5.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 42
Total number of positively charged residues (Arg + Lys): 37

Atomic composition:

Carbon      C	      1639
Hydrogen    H	      2615
Nitrogen    N	       443
Oxygen      O	       520
Sulfur      S	         7

Formula: C1639H2615N443O520S7
Total number of atoms: 5224

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    13535
Abs 0.1% (=1 g/l)   0.365, assuming all pairs of Cys residues form cystines


Ext. coefficient    13410
Abs 0.1% (=1 g/l)   0.362, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 48.18
This classifies the protein as unstable.



Aliphatic index: 87.50

Grand average of hydropathicity (GRAVY): -0.441