ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSTSHCRFYE NKYPEIDDIV MVNVQQIAEM GAYVKLLEYD NIEGMILLSE LSRRRIRSIQ 

        70         80         90        100        110        120 
KLIRVGKNDV AVVLRVDKEK GYIDLSKRRV SSEDIIKCEE KYQKSKTVHS ILRYCAEKFQ 

       130        140        150        160        170        180 
IPLEELYKTI AWPLSRKFGH AYEAFKLSII DETVWEGIEP PSKDVLDELK NYISKRLTPQ 

       190        200        210        220        230        240 
AVKIRADVEV SCFSYEGIDA IKDALKSAED MSTEQMQVKV KLVAAPLYVL TTQALDKQKG 

       250        260        270        280        290        300 
IEQLESAIEK ITEVITKYGG VCNITMPPKA VTATEDAELQ ALLESKELDN RSDSEDDEDE 


SDDE 

References and documentation are available.

Number of amino acids: 304

Molecular weight: 34717.66

Theoretical pI: 4.93

Amino acid composition: 
Ala (A)  20	  6.6%
Arg (R)  14	  4.6%
Asn (N)   7	  2.3%
Asp (D)  23	  7.6%
Cys (C)   5	  1.6%
Gln (Q)  12	  3.9%
Glu (E)  34	 11.2%
Gly (G)  10	  3.3%
His (H)   3	  1.0%
Ile (I)  26	  8.6%
Leu (L)  26	  8.6%
Lys (K)  29	  9.5%
Met (M)   7	  2.3%
Phe (F)   5	  1.6%
Pro (P)   9	  3.0%
Ser (S)  23	  7.6%
Thr (T)  13	  4.3%
Trp (W)   2	  0.7%
Tyr (Y)  13	  4.3%
Val (V)  23	  7.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 57
Total number of positively charged residues (Arg + Lys): 43

Atomic composition:

Carbon      C	      1533
Hydrogen    H	      2478
Nitrogen    N	       402
Oxygen      O	       487
Sulfur      S	        12

Formula: C1533H2478N402O487S12
Total number of atoms: 4912

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    30620
Abs 0.1% (=1 g/l)   0.882, assuming all pairs of Cys residues form cystines


Ext. coefficient    30370
Abs 0.1% (=1 g/l)   0.875, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 52.56
This classifies the protein as unstable.



Aliphatic index: 95.23

Grand average of hydropathicity (GRAVY): -0.421