ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSTSSQSFVA GRPASMASPS QSHRFCGPSA TASGGGSFDT LNRVIADLCS RGNPKEGAPL 

        70         80         90        100        110        120 
AFRKHVEEAV RDLSGEASSR FMEQLYDRIA NLIESTDVAE NMGALRAIDE LTEIGFGENA 

       130        140        150        160        170        180 
TKVSRFAGYM RTVFELKRDP EILVLASRVL GHLARAGGAM TSDEVEFQMK TAFDWLRVDR 

       190        200        210        220        230        240 
VEYRRFAAVL ILKEMAENAS TVFNVHVPEF VDAIWVALRD PQLQVRERAV EALRACLRVI 

       250        260        270        280        290        300 
EKRETRWRVQ WYYRMFEATQ DGLGRNAPVH SIHGSLLAVG ELLRNTGEFM MSRYREVAEI 

       310        320        330        340        350        360 
VLRYLEHRDR LVRLSITSLL PRIAHFLRDR FVTNYLTICM NHILTVLRIP AERASGFIAL 

       370        380        390        400        410        420 
GEMAGALDGE LIHYLPTIMS HLRDAIAPRK GRPLLEAVAC VGNIAKAMGS TVETHVRDLL 

       430        440        450        460        470        480 
DVMFSSSLSS TLVDALDQIT ISIPSLLPTV QDRLLDCISL VLSKSHYSQA KPPVTIVRGS 

       490        500        510        520        530        540 
TVGMAPQSSD PSCSAQVQLA LQTLARFNFK GHDLLEFARE SVVVYLDDED AATRKDAALC 

       550        560        570        580        590        600 
CCRLIANSLS GITQFGSSRS TRAGGRRRRL VEEIVEKLLR TAVADADVTV RKSIFVALFG 

       610        620        630        640        650        660 
NQCFDDYLAQ ADSLTAIFAS LNDEDLDVRE YAISVAGRLS EKNPAYVLPA LRRHLIQLLT 

       670        680        690        700        710        720 
YLELSADNKC REESAKLLGC LVRNCERLIL PYVAPVQKAL VARLSEGTGV NANNNIVTGV 

       730        740        750        760        770        780 
LVTVGDLARV GGLAMRQYIP ELMPLIVEAL MDGAAVAKRE VAVSTLGQVV QSTGYVVTPY 

       790        800        810        820        830        840 
KEYPLLLGLL LKLLKGDLVW STRREVLKVL GIMGALDPHV HKRNQQSLSG SHGEVPRGTG 

       850        860        870        880        890        900 
DSGQPIPSID ELPVELRPSF ATSEDYYSTV AINSLMRILR DASLLSYHKR VVRSLMIIFK 

       910        920        930        940        950        960 
SMGLGCVPYL PKVLPELFHT VRTSDENLKD FITWGLGTLV SIVRQHIRKY LPELLSLVSE 

       970        980        990       1000       1010       1020 
LWSSFTLPGP IRPSRGLPVL HLLEHLCLAL NDEFRTYLPV ILPCFIQVLG DAERFNDYTY 

      1030       1040       1050       1060       1070       1080 
VPDILHTLEV FGGTLDEHMH LLLPALIRLF KVDAPVAIRR DAIKTLTRVI PCVQVTGHIS 

      1090       1100       1110       1120       1130       1140 
ALVHHLKLVL DGKNDELRKD AVDALCCLAH ALGEDFTIFI ESIHKLLLKH RLRHKEFEEI 

      1150       1160       1170       1180       1190       1200 
HARWRRREPL IVATTATQQL SRRLPVEVIR DPVIENEIDP FEEGTDRNHQ VNDGRLRTAG 

      1210       1220       1230       1240       1250       1260 
EASQRSTKED WEEWMRHFSI ELLKESPSPA LRTCAKLAQL QPFVGRELFA AGFVSCWAQL 

      1270       1280       1290       1300       1310       1320 
NESSQKQLVR SLEMAFSSPN IPPEILATLL NLAEFMEHDE KPLPIDIRLL GALAEKCRVF 

      1330       1340       1350       1360       1370       1380 
AKALHYKEME FEGPRSKRMD ANPVAVVEAL IHINNQLHQH EAAVGILTYA QQHLDVQLKE 

      1390       1400       1410       1420       1430       1440 
SWYEKLQRWD DALKAYTLKA SQTTNPHLVL EATLGQMRCL AALARWEELN NLCKEYWSPA 

      1450       1460       1470       1480       1490       1500 
EPSARLEMAP MAAQAAWNMG EWDQMAEYVS RLDDGDETKL RGLASPVSSG DGSSNGTFFR 

      1510       1520       1530       1540       1550       1560 
AVLLVRRAKY DEAREYVERA RKCLATELAA LVLESYERAY SNMVRVQQLS ELEEVIEYYT 

      1570       1580       1590       1600       1610       1620 
LPVGNTIAEE RRALIRNMWT QRIQGSKRNV EVWQALLAVR ALVLPPTEDV ETWLKFASLC 

      1630       1640       1650       1660       1670       1680 
RKSGRISQAK STLLKLLPFD PEVSPENMQY HGPPQVMLGY LKYQWSLGEE RKRKEAFTKL 

      1690       1700       1710       1720       1730       1740 
QILTRELSSV PHSQSDILAS MVSSKGANVP LLARVNLKLG TWQWALSSGL NDGSIQEIRD 

      1750       1760       1770       1780       1790       1800 
AFDKSTCYAP KWAKAWHTWA LFNTAVMSHY ISRGQIASQY VVSAVTGYFY SIACAANAKG 

      1810       1820       1830       1840       1850       1860 
VDDSLQDILR LLTLWFNHGA TADVQTALKT GFSHVNINTW LVVLPQIIAR IHSNNRAVRE 

      1870       1880       1890       1900       1910       1920 
LIQSLLIRIG ENHPQALMYP LLVACKSISN LRRAAAQEVV DKVRQHSGAL VDQAQLVSHE 

      1930       1940       1950       1960       1970       1980 
LIRVAILWHE MWHEALEEAS RLYFGEHNIE GMLKVLEPLH DMLDEGVKKD STTIQERAFI 

      1990       2000       2010       2020       2030       2040 
EAYRHELKEA HECCCNYKIT GKDAELTQAW DLYYHVFKRI DKQLASLTTL DLESVSPELL 

      2050       2060       2070       2080       2090       2100 
LCRDLELAVP GTYRADAPVV TISSFSRQLV VITSKQRPRK LTIHGNDGED YAFLLKGHED 

      2110       2120       2130       2140       2150       2160 
LRQDERVMQL FGLVNTLLEN SRKTAEKDLS IQRYSVIPLS PNSGLIGWVP NCDTLHHLIR 

      2170       2180       2190       2200       2210       2220 
EHRDARKIIL NQENKHMLSF APDYDNLPLI AKVEVFEYAL ENTEGNDLSR VLWLKSRSSE 

      2230       2240       2250       2260       2270       2280 
VWLERRTNYT RSLAVMSMVG YILGLGDRHP SNLMLHRYSG KILHIDFGDC FEASMNREKF 

      2290       2300       2310       2320       2330       2340 
PEKVPFRLTR MLVKAMEVSG IEGNFRSTCE NVMQVLRTNK DSVMAMMEAF VHDPLINWRL 

      2350       2360       2370       2380       2390       2400 
FNFNEVPQLA LLGNNNPNAP ADVEPDEEDE DPADIDLPQP QRSTREKEIL QAVNMLGDAN 

      2410       2420       2430       2440       2450       2460 
EVLNERAVVV MARMSHKLTG RDFSSSAIPS NPIADHNNLL GGDSHEVEHG LSVKVQVQKL 

      2470       2480 
INQATSHENL CQNYVGWCPF W 

References and documentation are available.

Number of amino acids: 2481

Molecular weight: 279189.85

Theoretical pI: 6.37

Amino acid composition: 
Ala (A) 217	  8.7%
Arg (R) 182	  7.3%
Asn (N)  94	  3.8%
Asp (D) 123	  5.0%
Cys (C)  39	  1.6%
Gln (Q)  90	  3.6%
Glu (E) 182	  7.3%
Gly (G) 125	  5.0%
His (H)  75	  3.0%
Ile (I) 120	  4.8%
Leu (L) 315	 12.7%
Lys (K)  99	  4.0%
Met (M)  60	  2.4%
Phe (F)  77	  3.1%
Pro (P) 105	  4.2%
Ser (S) 176	  7.1%
Thr (T) 112	  4.5%
Trp (W)  37	  1.5%
Tyr (Y)  63	  2.5%
Val (V) 190	  7.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 305
Total number of positively charged residues (Arg + Lys): 281

Atomic composition:

Carbon      C	     12410
Hydrogen    H	     19791
Nitrogen    N	      3497
Oxygen      O	      3627
Sulfur      S	        99

Formula: C12410H19791N3497O3627S99
Total number of atoms: 39424

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   299745
Abs 0.1% (=1 g/l)   1.074, assuming all pairs of Cys residues form cystines


Ext. coefficient   297370
Abs 0.1% (=1 g/l)   1.065, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.78
This classifies the protein as unstable.



Aliphatic index: 99.33

Grand average of hydropathicity (GRAVY): -0.144