ProtParam
User-provided sequence:
10 20 30 40 50 60
MSTSTEKRSK ENLPWVEKYR PETLDEVYGQ NEVITTVRKF VDEGKLPHLL FYGPPGTGKT
70 80 90 100 110 120
STIVALAREI YGKNYSNMVL ELNASDDRGI DVVRNQIKDF ASTRQIFSKG FKLIILDEAD
130 140 150 160 170 180
AMTNAAQNAL RRVIERYTKN TRFCVLANYA HKLTPALLSR CTRFRFQPLP QEAIERRIAN
190 200 210 220 230 240
VLVHEKLKLS PNAEKALIEL SNGDMRRVLN VLQSCKATLD NPDEDEISDD VIYECCGAPR
250 260 270 280 290 300
PSDLKAVLKS ILEDDWGTAH YTLNKVRSAK GLALIDLIEG IVKILEDYEL QNEETRVHLL
310 320 330 340
TKLADIEYSI SKGGNDQIQG SAVIGAIKAS FENETVKANV
References and
documentation are available.
Number of amino acids: 340
Molecular weight: 38203.59
Theoretical pI: 6.07
Total number of negatively charged residues (Asp + Glu): 49
Total number of positively charged residues (Arg + Lys): 46
Atomic composition:
Carbon C 1687
Hydrogen H 2728
Nitrogen N 470
Oxygen O 520
Sulfur S 9
Formula: C
1687H
2728N
470O
520S
9
Total number of atoms: 5414
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 27640
Abs 0.1% (=1 g/l) 0.723, assuming all pairs of Cys residues form cystines
Ext. coefficient 27390
Abs 0.1% (=1 g/l) 0.717, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 38.49
This classifies the protein as stable.
Aliphatic index: 96.38
Grand average of hydropathicity (GRAVY): -0.357