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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSTSTEKRSK ENLPWVEKYR PETLDEVYGQ NEVITTVRKF VDEGKLPHLL FYGPPGTGKT

70 80 90 100 110 120
STIVALAREI YGKNYSNMVL ELNASDDRGI DVVRNQIKDF ASTRQIFSKG FKLIILDEAD

130 140 150 160 170 180
AMTNAAQNAL RRVIERYTKN TRFCVLANYA HKLTPALLSR CTRFRFQPLP QEAIERRIAN

190 200 210 220 230 240
VLVHEKLKLS PNAEKALIEL SNGDMRRVLN VLQSCKATLD NPDEDEISDD VIYECCGAPR

250 260 270 280 290 300
PSDLKAVLKS ILEDDWGTAH YTLNKVRSAK GLALIDLIEG IVKILEDYEL QNEETRVHLL

310 320 330 340
TKLADIEYSI SKGGNDQIQG SAVIGAIKAS FENETVKANV


References and documentation are available.

Number of amino acids: 340 Molecular weight: 38203.59 Theoretical pI: 6.07
Amino acid composition: 
Ala (A) 27 7.9% Arg (R) 21 6.2% Asn (N) 20 5.9% Asp (D) 21 6.2% Cys (C) 5 1.5% Gln (Q) 10 2.9% Glu (E) 28 8.2% Gly (G) 17 5.0% His (H) 5 1.5% Ile (I) 24 7.1% Leu (L) 36 10.6% Lys (K) 25 7.4% Met (M) 4 1.2% Phe (F) 9 2.6% Pro (P) 12 3.5% Ser (S) 20 5.9% Thr (T) 20 5.9% Trp (W) 2 0.6% Tyr (Y) 11 3.2% Val (V) 23 6.8% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 49 Total number of positively charged residues (Arg + Lys): 46 Atomic composition: Carbon C 1687 Hydrogen H 2728 Nitrogen N 470 Oxygen O 520 Sulfur S 9 Formula: C1687H2728N470O520S9 Total number of atoms: 5414 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 27640 Abs 0.1% (=1 g/l) 0.723, assuming all pairs of Cys residues form cystines Ext. coefficient 27390 Abs 0.1% (=1 g/l) 0.717, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 38.49 This classifies the protein as stable. Aliphatic index: 96.38 Grand average of hydropathicity (GRAVY): -0.357