ProtParam
User-provided sequence:
10 20 30 40 50 60
MSTTLLRTIQ LNQFSLLGTS LLRRRRSNNF SVRSCGSQTT TTHEAKQSSP TRVAPKNHKS
70 80 90 100 110 120
NQLDEILRDY EAVIGIETHV QLSTLTKAFC SCSNNYGSYP NTSICPVCMG LPGALPVLNS
130 140 150 160 170 180
KVVEFGVRLG LALNCDLSLK SKFDRKQYFY PDLPKGYQIS QFDIPIASGG YVDVDIPLEF
190 200 210 220 230 240
GGGHRRFGIT RVHMEEDAGK LLHSDTGDYS QVDLNRAGVP LLEIVSEPDM RSGIEAAEYA
250 260 270 280 290 300
CEMQRIARYL GVSNGNMQEG SLRCDVNISI RPIGQAEFGT KVEIKNLNAF SAISRAIDFE
310 320 330 340 350 360
ISRQALLYNQ GKADQIVTET RLWEEGAQKT VTMRKKEGLA DYRYFPEPDL PEVILTQEYV
370 380 390 400 410 420
DSIRASLPEL PEAKRRRYEA MGLGMQDVLF LANDVSVAEY FDAVIGKGAE VKLAANWIMS
430 440 450 460 470 480
DIAAYLKNEK LSINDIKLTP QELAELIAAI KDGTISGKIG KEILFELLAK GGTVKGMIKA
490 500 510 520 530 540
KDLVQITDPA EIEKMVIQVV SENPKQLEQY RSGKTKLQGY FAGQVMKMSK GKANPGLLNK
550
ILLEKLNAKD
References and
documentation are available.
Number of amino acids: 550
Molecular weight: 60940.93
Theoretical pI: 7.52
Total number of negatively charged residues (Asp + Glu): 67
Total number of positively charged residues (Arg + Lys): 68
Atomic composition:
Carbon C 2694
Hydrogen H 4345
Nitrogen N 741
Oxygen O 820
Sulfur S 22
Formula: C
2694H
4345N
741O
820S
22
Total number of atoms: 8622
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 39810
Abs 0.1% (=1 g/l) 0.653, assuming all pairs of Cys residues form cystines
Ext. coefficient 39310
Abs 0.1% (=1 g/l) 0.645, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 42.63
This classifies the protein as unstable.
Aliphatic index: 92.22
Grand average of hydropathicity (GRAVY): -0.270