ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSVCTLLISC AILAAPTLGS LQERRLYEDL MRNYNNLERP VANHSEPVTV HLKVALQQII 

        70         80         90        100        110        120 
DVDEKNQVVY VNAWLDYTWN DYNLVWDKAE YGNITDVRFP AGKIWKPDVL LYNSVDTNFD 

       130        140        150        160        170        180 
STYQTNMIVY STGLVHWVPP GIFKISCKID IQWFPFDEQK CFFKFGSWTY DGYKLDLQPA 

       190        200        210        220        230        240 
TGGFDISEYI SNGEWALPLT TVERNEKFYD CCPEPYPDVH FYLHMRRRTL YYGFNLIMPC 

       250        260        270        280        290        300 
ILTTLMTLLG FTLPPDAGEK ITLQITVLLS ICFFLSIVSE MSPPTSEAVP LLGIFFTCCM 

       310        320        330        340        350        360 
IVVTASTVFT VYVLNLHYRT PETHDMGPWT RNLLLYWIPW ILRMKRPGHN LTYASLPSLF 

       370        380        390        400        410        420 
STKPNRHSES LIRNIKDNEH SLSRANSFDA DCRLNQYIMT QSVSNGLTSL GSIPSTMISS 

       430        440        450        460        470        480 
NGTTTDVSQQ ATLLILHRIY HELKIVTKRM IEGDKEEQAC NNWKFAAMVR TYISRKIENF 

References and documentation are available.

Number of amino acids: 480

Molecular weight: 55274.61

Theoretical pI: 6.07

Amino acid composition: 
Ala (A)  20	  4.2%
Arg (R)  21	  4.4%
Asn (N)  28	  5.8%
Asp (D)  24	  5.0%
Cys (C)  12	  2.5%
Gln (Q)  14	  2.9%
Glu (E)  23	  4.8%
Gly (G)  20	  4.2%
His (H)  12	  2.5%
Ile (I)  34	  7.1%
Leu (L)  51	 10.6%
Lys (K)  20	  4.2%
Met (M)  14	  2.9%
Phe (F)  22	  4.6%
Pro (P)  26	  5.4%
Ser (S)  34	  7.1%
Thr (T)  39	  8.1%
Trp (W)  12	  2.5%
Tyr (Y)  24	  5.0%
Val (V)  30	  6.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 47
Total number of positively charged residues (Arg + Lys): 41

Atomic composition:

Carbon      C	      2511
Hydrogen    H	      3849
Nitrogen    N	       641
Oxygen      O	       714
Sulfur      S	        26

Formula: C2511H3849N641O714S26
Total number of atoms: 7741

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   102510
Abs 0.1% (=1 g/l)   1.855, assuming all pairs of Cys residues form cystines


Ext. coefficient   101760
Abs 0.1% (=1 g/l)   1.841, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.89
This classifies the protein as unstable.



Aliphatic index: 91.35

Grand average of hydropathicity (GRAVY): -0.086