ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSVEDIKKAR AAVPFNREQL ESVLRGRFFY APAFDLYGGV SGLYDYGPPG CAFQNNIIDA 

        70         80         90        100        110        120 
WRKHFILEED MLEVDCTMLT PYEVLKTSGH VDKFSDWMCR DLKTGEIFRA DHLVEEVLEA 

       130        140        150        160        170        180 
RLKGDQEARG LVEDANAAAK DDAEKKKRKK KVKQIKAVKL DDDVVKEYEE ILAKIDGYSG 

       190        200        210        220        230        240 
PELGELMEKY DIGNPVTGET LESPRAFNLM FETAIGPSGQ LKGYLRPETA QGQFLNFNKL 

       250        260        270        280        290        300 
LEFNNSKTPF ASASIGKSFR NEISPRAGLL RVREFLMAEI EHFVDPLDKS HPKFNEIKDI 

       310        320        330        340        350        360 
KLSFLPRDVQ EAGSTEPIVK TVGEAVASRM VDNETLGYFI ARIYQFLMKI GVDESKLRFR 

       370        380        390        400        410        420 
QHMANEMAHY AADCWDGELK TSYGWIECVG CADRSAYDLT VHSKKTKEKL VVRQKLDNPI 

       430        440        450        460        470        480 
EVTKWEIDLT KKLFGPKFRK DAPKVESHLL NMSQDDLASK AELLKANGKF TIKVDGVDGE 

       490        500        510        520        530        540 
VELDDKLVKI EQRTKVEHVR EYVPSVIEPS FGIGRIIYSV FEHSFWNRPE DNARSVLSFP 

       550        560        570        580        590        600 
PLVAPTKVLL VPLSNHKDLV PVTTEVAKIL RKSQIPFKID DSGVSIGKRY ARNDELGTPF 

       610        620        630        640        650        660 
GVTIDFESAK DHSVTLRERD STKQVRGSVE NVIKAIRDIT YNGASWEEGT KDLTPFIAQA 


EAEAETD 

References and documentation are available.

Number of amino acids: 667

Molecular weight: 75338.82

Theoretical pI: 5.63

Amino acid composition: 
Ala (A)  49	  7.3%
Arg (R)  36	  5.4%
Asn (N)  23	  3.4%
Asp (D)  49	  7.3%
Cys (C)   6	  0.9%
Gln (Q)  16	  2.4%
Glu (E)  61	  9.1%
Gly (G)  41	  6.1%
His (H)  13	  1.9%
Ile (I)  37	  5.5%
Leu (L)  56	  8.4%
Lys (K)  60	  9.0%
Met (M)  12	  1.8%
Phe (F)  33	  4.9%
Pro (P)  30	  4.5%
Ser (S)  39	  5.8%
Thr (T)  30	  4.5%
Trp (W)   7	  1.0%
Tyr (Y)  18	  2.7%
Val (V)  51	  7.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 110
Total number of positively charged residues (Arg + Lys): 96

Atomic composition:

Carbon      C	      3370
Hydrogen    H	      5315
Nitrogen    N	       907
Oxygen      O	      1014
Sulfur      S	        18

Formula: C3370H5315N907O1014S18
Total number of atoms: 10624

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    65695
Abs 0.1% (=1 g/l)   0.872, assuming all pairs of Cys residues form cystines


Ext. coefficient    65320
Abs 0.1% (=1 g/l)   0.867, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 34.35
This classifies the protein as stable.



Aliphatic index: 83.90

Grand average of hydropathicity (GRAVY): -0.440