ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSVEEGMEVE LKSEKMDLDD NIPDFVKETV ERSGKNPMSL FSELYVHMTG NTPVFDFYNR 

        70         80         90        100        110        120 
NQPNGSMKFI CVVILNGERI EGNVKSKKKE AKVSCSLKGL EVVLKHVSEY VVPAVKFEEK 

       130        140        150        160        170        180 
TTFFEMLREH TYAKFYELCK NNALIYGFEK VIASVFLKIN GNLQIIALST GNKGLRGDKI 

       190        200        210        220        230        240 
VNDGTALIDC HAEILARRGL LRFLYSEVLK FSTEPPNSIF TKGKNALVLK PGISFHLFIN 

       250        260        270        280        290        300 
TAPCGVARID KKLKPGTSDD LQNSSRLRFK IDKGMGTVLG GASEFEAPQT FDGIMMGERM 

       310        320        330        340        350        360 
RTMSCSDKLL RANVLGVQGA ILSHFIDPIY YSSIAVAELN NADRLRKAVY SRAATFKPPA 

       370        380        390        400        410        420 
PFHVQDVEIG ECQVEDTEQS TSAAARSTIS SMNWNLADGN TEVVRTSDGM VHDKDMSGAD 

       430        440        450        460        470        480 
ITTPSRLCKK NMAELMITIC TLTKTSVDYP ISYEELKAGS QEYAAAKKSF ITWLRQKDLG 

       490 
IWQRKPREFQ MFTIN 

References and documentation are available.

Number of amino acids: 495

Molecular weight: 55319.66

Theoretical pI: 8.21

Amino acid composition: 
Ala (A)  33	  6.7%
Arg (R)  23	  4.6%
Asn (N)  26	  5.3%
Asp (D)  25	  5.1%
Cys (C)   9	  1.8%
Gln (Q)  12	  2.4%
Glu (E)  36	  7.3%
Gly (G)  32	  6.5%
His (H)   8	  1.6%
Ile (I)  31	  6.3%
Leu (L)  41	  8.3%
Lys (K)  41	  8.3%
Met (M)  18	  3.6%
Phe (F)  25	  5.1%
Pro (P)  18	  3.6%
Ser (S)  38	  7.7%
Thr (T)  29	  5.9%
Trp (W)   3	  0.6%
Tyr (Y)  13	  2.6%
Val (V)  34	  6.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 61
Total number of positively charged residues (Arg + Lys): 64

Atomic composition:

Carbon      C	      2453
Hydrogen    H	      3912
Nitrogen    N	       662
Oxygen      O	       736
Sulfur      S	        27

Formula: C2453H3912N662O736S27
Total number of atoms: 7790

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    36370
Abs 0.1% (=1 g/l)   0.657, assuming all pairs of Cys residues form cystines


Ext. coefficient    35870
Abs 0.1% (=1 g/l)   0.648, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 34.41
This classifies the protein as stable.



Aliphatic index: 83.31

Grand average of hydropathicity (GRAVY): -0.249