ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSVFESETSN FHVYNNHEIQ TQPQMQTFLS EEEPVGRQNS ILSLTLDEIQ MKSGKSFGAM 

        70         80         90        100        110        120 
NMDEFLANLW TTVEENDNEG GGAHNDGEKP AVLPRQGSLS LPVPLCKKTV DEVWLEIQNG 

       130        140        150        160        170        180 
VQQHPPSSNS GQNSAENIRR QQTLGEITLE DFLVKAGVVQ EPLKTTMRMS SSDFGYNPEF 

       190        200        210        220        230        240 
GVGLHCQNQN NYGDNRSVYS ENRPFYSVLG ESSSCMTGNG RSNQYLTGLD AFRIKKRIID 

       250        260        270        280        290        300 
GPPEILMERR QRRMIKNRES AARSRARRQA YTVELELELN NLTEENTKLK EIVEENEKKR 

       310        320        330 
RQEIISRSKQ VTKEKSGDKL RKIRRMASAG W 

References and documentation are available.

Number of amino acids: 331

Molecular weight: 37665.14

Theoretical pI: 6.30

Amino acid composition: 
Ala (A)  13	  3.9%
Arg (R)  25	  7.6%
Asn (N)  26	  7.9%
Asp (D)  11	  3.3%
Cys (C)   3	  0.9%
Gln (Q)  21	  6.3%
Glu (E)  35	 10.6%
Gly (G)  23	  6.9%
His (H)   5	  1.5%
Ile (I)  15	  4.5%
Leu (L)  26	  7.9%
Lys (K)  19	  5.7%
Met (M)  11	  3.3%
Phe (F)  10	  3.0%
Pro (P)  13	  3.9%
Ser (S)  30	  9.1%
Thr (T)  17	  5.1%
Trp (W)   3	  0.9%
Tyr (Y)   7	  2.1%
Val (V)  18	  5.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 46
Total number of positively charged residues (Arg + Lys): 44

Atomic composition:

Carbon      C	      1616
Hydrogen    H	      2593
Nitrogen    N	       485
Oxygen      O	       525
Sulfur      S	        14

Formula: C1616H2593N485O525S14
Total number of atoms: 5233

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    27055
Abs 0.1% (=1 g/l)   0.718, assuming all pairs of Cys residues form cystines


Ext. coefficient    26930
Abs 0.1% (=1 g/l)   0.715, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 61.63
This classifies the protein as unstable.



Aliphatic index: 68.01

Grand average of hydropathicity (GRAVY): -0.858