ProtParam
User-provided sequence:
10 20 30 40 50 60
MSVHAATNPI NKHVVLIDNY DSFTWNVYEY LCQEGAKVSV YRNDAITVPE IAALNPDTLL
70 80 90 100 110 120
ISPGPGHPKT DSGISRDCIR YFTGKIPVFG ICMGQQCMFD VFGGEVAYAG EIVHGKTSPI
130 140 150 160 170 180
SHDNCGIFKN VPQGIAVTRY HSLAGTESSL PSCLKVTAST ENGIIMGVRH KKYTVEGVQF
190 200 210 220 230 240
HPESILTEEG HLMIRNILNV SGGTWEENKS SPSNSILDRI YARRKIDVNE QSKIPGFTFQ
250 260 270 280 290 300
DLQSNYDLGL APPLQDFYTV LSSSHKRAVV LAEVKRASPS KGPICLKAVA AEQALKYAEA
310 320 330 340 350 360
GASAISVLTE PHWFHGSLQD LVNVRKILDL KFPPKERPCV LRKEFIFSKY QILEARLAGA
370 380 390 400 410 420
DTVLLIVKML SQPLLKELYS YSKDLNMEPL VEVNSKEELQ RALEIGAKVV GVNNRDLHSF
430 440 450 460 470 480
NVDLNTTSNL VESIPKDVLL IALSGITTRD DAEKYKKEGV HGFLVGEALM KSTDVKKFIH
ELCE
References and
documentation are available.
Number of amino acids: 484
Molecular weight: 53489.38
Theoretical pI: 6.44
Total number of negatively charged residues (Asp + Glu): 56
Total number of positively charged residues (Arg + Lys): 52
Atomic composition:
Carbon C 2392
Hydrogen H 3800
Nitrogen N 642
Oxygen O 712
Sulfur S 17
Formula: C
2392H
3800N
642O
712S
17
Total number of atoms: 7563
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 40840
Abs 0.1% (=1 g/l) 0.764, assuming all pairs of Cys residues form cystines
Ext. coefficient 40340
Abs 0.1% (=1 g/l) 0.754, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 41.82
This classifies the protein as unstable.
Aliphatic index: 95.64
Grand average of hydropathicity (GRAVY): -0.138