ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSVHGRDPKK RQLRLISVAF KEASIDSPSF RASVNFFQTR VDALEDWIEK TVDFFDQKYK 

        70         80         90        100        110        120 
VSFEDFRRAK ETLLSQLLPP PALLSNGFVS NQSFTPRLID SFNKDYYDFS MKLLQIVKGD 

       130        140        150        160        170        180 
DSSHSTALLE LMTTAIEPYR NVRKNFDFYQ GKYDSMLASY QAIRISKTSL EPSSIKSDAL 

       190        200        210        220        230        240 
QLFEVQKNYL KASLDLISAI SAVKLSLDKF ILESMKVLKS RSIFITKDSG RKIDLSPCIN 

       250        260        270        280        290        300 
EYLDNYAIWV ENSIEGSKVL DSDISNAKKQ AYRYTLKRIT PSSDTSDYNI RSIHSSKLLS 

       310        320        330        340        350        360 
KDTQVPPKSP EKSGWLYMKT QVGKPTREIW VRRWCFLKNA VFGMFLLSPS KTYVEETDKF 

       370        380        390        400        410        420 
GVFLTNVRYD PEEDRKFCFE VKIFGNKVTE AHDNMSKDIT LVFQTSNYLD LKSWLIAFEA 

       430        440        450        460        470        480 
TKKYVMSIQH DSLEYELAFK RFSPKFFEFA SSTTTSIDQL ITTFDKETES LYETLNCSIS 

       490        500        510        520        530        540 
EYDILTLGEE KVFQFQMPTT PISTKMTQLA ILSNFLTKGS WFPNAVLANI WGTTDWSEYT 

       550        560        570        580        590        600 
ILPGKGKKPS SLLTIDGKRL PIRNSTIYPQ YYSNELKVLD LQFKSLVFSP DQRLEKLPEE 

       610        620        630        640        650        660 
LLLFKFEALW CPNKKQKFSA TCFCTKDYIY CYMNSMEFIC LTKISLSEIV SVEADRSSKK 

       670        680        690        700        710        720 
TLKLYDASGL QMKAIVLFSD YKLIASKLQY LLENKAIKNP NSNEEILVKF EQMEKESQEK 

       730        740        750        760        770        780 
KQEELYKIEQ ENSFDRKATS VSKIIKSRVT FWEMSDDAST LLNRLKKLQT EYSITYNHEY 

       790        800        810        820        830        840 
EISSKGLAHI LFGDKSNAFP KCLFLARKDG EEHGKRFWYK NKDINGKSQL VRKIPFRLDM 

       850        860        870        880        890        900 
TGNFLNTGKY HRDKESKMIF ATQRIVKIVD NKYYEVDLDP FFVKVPFCHL LKLSIKFVIT 

       910        920        930        940        950        960 
ESYDVDNHLE IKLNMTASSS SLHVLYKLEY IDSRTGKTIE KLSLAEIICQ TWALKFAHSE 

       970        980        990       1000       1010       1020 
FLLIRRVLRY YLEKIGKHGK VIKAIKLCGI LGVLSNKSEE PATEKNGNSK ESESMQYDIR 

      1030       1040       1050       1060       1070       1080 
YSCTILFLVF IKLMVYRVTN LTFVFFRILI GILLLCAEKF SRINRMMVVG LLASIMINIL 

      1090       1100       1110       1120       1130       1140 
LSEKASVPYW SIKRAEKLFH DRLGSDKFTM QRAIYISDSD LLSSQLSVPS NNPIFEKFSE 

      1150       1160       1170       1180       1190       1200 
DNFNKDYQYS ETRKQLAMRR NELLIELRIL QDMEKQLVHD DYEKFLLEEV NKCSMVSIEM 

      1210       1220 
TDLWFNDTQL QNYCSICNEE LEKLRPPIT 

References and documentation are available.

Number of amino acids: 1229

Molecular weight: 142819.79

Theoretical pI: 8.79

Amino acid composition: 
Ala (A)  51	  4.1%
Arg (R)  52	  4.2%
Asn (N)  55	  4.5%
Asp (D)  69	  5.6%
Cys (C)  18	  1.5%
Gln (Q)  42	  3.4%
Glu (E)  87	  7.1%
Gly (G)  34	  2.8%
His (H)  16	  1.3%
Ile (I)  88	  7.2%
Leu (L) 137	 11.1%
Lys (K) 122	  9.9%
Met (M)  29	  2.4%
Phe (F)  74	  6.0%
Pro (P)  38	  3.1%
Ser (S) 125	 10.2%
Thr (T)  66	  5.4%
Trp (W)  15	  1.2%
Tyr (Y)  53	  4.3%
Val (V)  58	  4.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 156
Total number of positively charged residues (Arg + Lys): 174

Atomic composition:

Carbon      C	      6478
Hydrogen    H	     10173
Nitrogen    N	      1651
Oxygen      O	      1883
Sulfur      S	        47

Formula: C6478H10173N1651O1883S47
Total number of atoms: 20232

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   162595
Abs 0.1% (=1 g/l)   1.138, assuming all pairs of Cys residues form cystines


Ext. coefficient   161470
Abs 0.1% (=1 g/l)   1.131, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.04
This classifies the protein as unstable.



Aliphatic index: 89.24

Grand average of hydropathicity (GRAVY): -0.317