ProtParam
User-provided sequence:
10 20 30 40 50 60
MSVPNSKEQS LLDDASTLLL FSKGKKRAEE ASKIGSKTDT IEHDESHERE KKGAIEMAAA
70 80 90 100 110 120
ALATASTVSL PLKKATEQSA AEAATSTAAK EETENQPQKQ PQWPVPDSYI VDPDAGIITC
130 140 150 160 170 180
ICDLNDDDGF TIQCDHCNRW QHAICYGIKD IGMAPDDYLC NSCDPREVDI NLARKIQQER
190 200 210 220 230 240
INVKTVEPSS SNNSASNKNN GRDRASSTTI SDVGDSFSTD QDNTNHRDKR RKRNPSNNSI
250 260 270 280 290 300
DSKNESASVN SSDGLTSMPK KKEHFLSAKD AYGAIYLPLK DNVFKSDLIE PFLNKHMDDN
310 320 330 340 350 360
WVIQYPHKTF KSVSIEVKPY ADIAYSRTYP GFTKLGVYLK KDCIKGDFIQ EILGELDFYK
370 380 390 400 410 420
NYLTDPRNHY RIWGTAKRRV IFHSHWPIYI DARLSGNSTR YLRRSCQPNV ELVTIKLQDT
430 440 450 460 470 480
DNRNDKSSGR KSSRIKFVLR ALRDISEDEE LYIKWQWDSK HPILKLIKGM TIDSLDDLER
490 500 510 520 530 540
YGLINSVETI LSNGECGCGN NSKDCYLLKV KRYAQSLYKS VKSRGKMNNR YKLNEILNQY
550 560 570 580 590 600
NCKKRREPPI LHRLEEKAQN TIERAPILLN NFYRQKFLNR NNGPKIPQKN TIDSTNNPDD
610 620 630 640 650 660
IAKPFKFALF AQHSSNISVP KKNETSEKPL IITKSTDYDE SHITNIEELP IPVLLPINKT
670 680 690 700 710 720
SRQTANDVEE SQSKNEHKLS RTPSLSNFNK ELSKEAQHSQ AKTKEIMTEA SVNSRRESTP
730 740 750
ESIMHLSDFS SSQLHSKKKL SFADYRKKLL K
References and
documentation are available.
Number of amino acids: 751
Molecular weight: 85480.13
Theoretical pI: 8.90
Total number of negatively charged residues (Asp + Glu): 102
Total number of positively charged residues (Arg + Lys): 116
Atomic composition:
Carbon C 3729
Hydrogen H 5956
Nitrogen N 1076
Oxygen O 1182
Sulfur S 22
Formula: C
3729H
5956N
1076O
1182S
22
Total number of atoms: 11965
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 76500
Abs 0.1% (=1 g/l) 0.895, assuming all pairs of Cys residues form cystines
Ext. coefficient 75750
Abs 0.1% (=1 g/l) 0.886, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 47.32
This classifies the protein as unstable.
Aliphatic index: 73.02
Grand average of hydropathicity (GRAVY): -0.840