ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSVQKMMWVP RKMVGGRIPF FTCSKVFSGF SRRSFHESPL ARSTYEEEKV LVDEIKQKLT 

        70         80         90        100        110        120 
PDDIGRCNKL RNIGISAHID SGKTTFTERV LYYTKRIKAI HEVRGRDNVG AKMDSMDLER 

       130        140        150        160        170        180 
EKGITIQSAA TYCSWDKEGK NYHFNLIDTP GHIDFTIEVE RALRVLDGAV LVVCAVSGVQ 

       190        200        210        220        230        240 
SQTVTVDRQM RRYNVPRVTF INKMDRMGSD PFRAIEQLNS KLKIPAAAVQ IPIGSESSLS 

       250        260        270        280        290        300 
GVVDLINRVA IYNKGDNGEI IEKGPVPENL KPLMEEKRQL LIETLADVDD EMAEMFLEEK 

       310        320        330        340        350        360 
EPTTQQIKDA IRRSTIARSF TPVLMGSALA NTGIQPVLDA IVDYLPNPSE VLNTALDVSN 

       370        380        390        400        410        420 
NEAKVNLVPA VQQPFVGLAF KLEEGKYGQL TYVRVYQGRL RKGNYITNVK TGKKVKVARL 

       430        440        450        460        470        480 
VRMHSSEMED VDEVGSGEIC ATFGIDCASG DTFTDGSVQY SMSSMYVPDA VVSLSITPNS 

       490        500        510        520        530        540 
KDASNFSKAL NRFQKEDPTF RVKFDPESKE TIISGMGELH LEIYVERMRR EYNVDCVTGK 

       550        560        570        580        590        600 
PQVSYRESIT IPADFDYTHK KQSGGAGQYG RVIGTLSPVD DITKGNIFET AIVGGRIPDK 

       610        620        630        640        650        660 
YLAACGKGFE EVCEKGPLIG HRVLDVKMLI NDGAIHAVDS NELSFKTATM SAFRDAFLRA 

       670        680        690        700        710        720 
QPVIMEPIMN VSVTSPNEFQ GNVIGLLNKL QAVIQDTENG HDEFTLKAEC ALSTMFGFAT 

       730        740        750        760 
SLRASTQGKG EFSLEFSHYA PTAPHVQKEL ISEFQKKQAK K 

References and documentation are available.

Number of amino acids: 761

Molecular weight: 84573.62

Theoretical pI: 6.42

Amino acid composition: 
Ala (A)  50	  6.6%
Arg (R)  42	  5.5%
Asn (N)  32	  4.2%
Asp (D)  44	  5.8%
Cys (C)  10	  1.3%
Gln (Q)  29	  3.8%
Glu (E)  55	  7.2%
Gly (G)  54	  7.1%
His (H)  13	  1.7%
Ile (I)  50	  6.6%
Leu (L)  51	  6.7%
Lys (K)  53	  7.0%
Met (M)  24	  3.2%
Phe (F)  33	  4.3%
Pro (P)  33	  4.3%
Ser (S)  55	  7.2%
Thr (T)  45	  5.9%
Trp (W)   2	  0.3%
Tyr (Y)  21	  2.8%
Val (V)  65	  8.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 99
Total number of positively charged residues (Arg + Lys): 95

Atomic composition:

Carbon      C	      3729
Hydrogen    H	      5963
Nitrogen    N	      1029
Oxygen      O	      1142
Sulfur      S	        34

Formula: C3729H5963N1029O1142S34
Total number of atoms: 11897

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    42915
Abs 0.1% (=1 g/l)   0.507, assuming all pairs of Cys residues form cystines


Ext. coefficient    42290
Abs 0.1% (=1 g/l)   0.500, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.38
This classifies the protein as unstable.



Aliphatic index: 83.10

Grand average of hydropathicity (GRAVY): -0.304