ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSVSARSAAA EERSVNCSTM AQPKNLEGYV GFANLPNQVY RKSVKRGFEF TLMVVGESGL 

        70         80         90        100        110        120 
GKSTLINSLF LTDLYSPEYP GPSHRIKKTV QVEQSKVLIK EGGVQLLLTI VDTPGFGDAV 

       130        140        150        160        170        180 
DNSNCWQPVI DYIDSKFEDY LNAESRVNRR QMPDNRVQCC LYFIAPSGHG LKPLDIEFMK 

       190        200        210        220        230        240 
RLHEKVNIIP LIAKADTLTP EECQQFKKQI MKEIQGHKIK IYEFPETDDE EENKLVKKIK 

       250        260        270        280        290        300 
DRLPLAVVGS NTIIEVNGKR VRGRQYPWGV AEVENGEHCD FTILRNMLIR THMQDLKDVT 

       310        320        330        340        350        360 
NNVHYENYRS RKLAAVTYNG VDNNKNKGQL TKSPLAQMEE ERREHVAKMK KMEMEMEQVF 

       370        380        390        400        410        420 
EMKVKEKVQK LKDSEAELQR RHEQMKKNLE AQHKELEEKR RQFEEEKANW EAQQRILEQQ 

       430 
NSSRTLEKNK KKGKIF 

References and documentation are available.

Number of amino acids: 436

Molecular weight: 50507.82

Theoretical pI: 8.82

Amino acid composition: 
Ala (A)  21	  4.8%
Arg (R)  26	  6.0%
Asn (N)  27	  6.2%
Asp (D)  18	  4.1%
Cys (C)   6	  1.4%
Gln (Q)  26	  6.0%
Glu (E)  47	 10.8%
Gly (G)  22	  5.0%
His (H)  10	  2.3%
Ile (I)  22	  5.0%
Leu (L)  34	  7.8%
Lys (K)  46	 10.6%
Met (M)  15	  3.4%
Phe (F)  14	  3.2%
Pro (P)  16	  3.7%
Ser (S)  22	  5.0%
Thr (T)  17	  3.9%
Trp (W)   3	  0.7%
Tyr (Y)  12	  2.8%
Val (V)  32	  7.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 65
Total number of positively charged residues (Arg + Lys): 72

Atomic composition:

Carbon      C	      2214
Hydrogen    H	      3570
Nitrogen    N	       636
Oxygen      O	       671
Sulfur      S	        21

Formula: C2214H3570N636O671S21
Total number of atoms: 7112

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    34755
Abs 0.1% (=1 g/l)   0.688, assuming all pairs of Cys residues form cystines


Ext. coefficient    34380
Abs 0.1% (=1 g/l)   0.681, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 47.95
This classifies the protein as unstable.



Aliphatic index: 76.19

Grand average of hydropathicity (GRAVY): -0.781