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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSVSLEQTLG FRIKVTNVLD VVTEGRLYSF NSSNNTLTIQ TTKKNQSPQN FKVIKCTFIK

70 80 90 100 110 120
HLEVIGDKPS FNSFKKQQIK PSYVNVERVE KLLKESVIAS KKKELLRGKG VSAEGQFIFD

130 140 150 160 170 180
QIFKTIGDTK WVAKDIIILD DVKVQPPYKV EDIKVLHEGS NQSITLIQRI VERSWEQLEQ


DDGRKGG


References and documentation are available.

Number of amino acids: 187 Molecular weight: 21313.56 Theoretical pI: 9.41
Amino acid composition: 
Ala (A) 3 1.6% Arg (R) 7 3.7% Asn (N) 9 4.8% Asp (D) 10 5.3% Cys (C) 1 0.5% Gln (Q) 13 7.0% Glu (E) 13 7.0% Gly (G) 11 5.9% His (H) 2 1.1% Ile (I) 17 9.1% Leu (L) 14 7.5% Lys (K) 23 12.3% Met (M) 1 0.5% Phe (F) 9 4.8% Pro (P) 5 2.7% Ser (S) 15 8.0% Thr (T) 11 5.9% Trp (W) 2 1.1% Tyr (Y) 3 1.6% Val (V) 18 9.6% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 23 Total number of positively charged residues (Arg + Lys): 30 Atomic composition: Carbon C 957 Hydrogen H 1555 Nitrogen N 259 Oxygen O 285 Sulfur S 2 Formula: C957H1555N259O285S2 Total number of atoms: 3058 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 15470 Abs 0.1% (=1 g/l) 0.726, assuming all pairs of Cys residues form cystines Ext. coefficient 15470 Abs 0.1% (=1 g/l) 0.726, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 26.18 This classifies the protein as stable. Aliphatic index: 94.17 Grand average of hydropathicity (GRAVY): -0.442