ProtParam
User-provided sequence:
10 20 30 40 50 60
MSVSYKGTIS KTHSVFKPEK GRYYIYGALG CPFTHRAILA RSLKKLEPVL GLVLSHWQLD
70 80 90 100 110 120
SKGARFLPAP HRPEKYKERF FTATGGIASA KLDESEELGD VNNDSARLFV DGAFDPVENI
130 140 150 160 170 180
SRLSELYYLN DPKYPGTKFT VPVLWDSKTR KIVNNESGDI IRILNSGVFD EFIQSEETNV
190 200 210 220 230 240
IDLVPHDLID EIDKNIKWVH PKINLGVYKV GLAENGKIYE TEVKTLFENL QKMECVLKEN
250 260 270 280 290 300
YKRLEEQFSG NKQKILAKYF VLGQRLTEAD IRLYPSIIRF DVVYVQHFKC NLKTIRDGFP
310 320 330 340 350
YLHLWLINLY WNYAEFRFTT DFNHIKLFYI RMEVSRNKIN QFGIVPLGPK PDISRL
References and
documentation are available.
Number of amino acids: 356
Molecular weight: 41300.58
Theoretical pI: 8.91
Total number of negatively charged residues (Asp + Glu): 44
Total number of positively charged residues (Arg + Lys): 50
Atomic composition:
Carbon C 1898
Hydrogen H 2939
Nitrogen N 495
Oxygen O 526
Sulfur S 6
Formula: C
1898H
2939N
495O
526S
6
Total number of atoms: 5864
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 54445
Abs 0.1% (=1 g/l) 1.318, assuming all pairs of Cys residues form cystines
Ext. coefficient 54320
Abs 0.1% (=1 g/l) 1.315, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 38.07
This classifies the protein as stable.
Aliphatic index: 93.60
Grand average of hydropathicity (GRAVY): -0.329