ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSVTGPFSHP TNLNPTAPAF FPAINQHQNQ NPSLIPTRFF LPHPPPPPPP PPPPLYFSYF 

        70         80         90        100        110        120 
SLPPPPPPPH LPPTSVTPTR AVMLLQVPAT VTETSLRRDM ELFGEVRGVQ MERAHEGIVI 

       130        140        150        160        170        180 
FHFYNLINSQ RAFNEIRYRH MQQQEQQQHF HFTTARGLVS GHSLWAHFVF PQLNAVPEGN 

       190        200        210        220        230        240 
NQGSLVIMNL EPTVSSSTLR HIFQVYGEVK QVRETPCKRE QRFVEFFDVR DAAKALRVMN 

       250        260        270        280        290        300 
GKVISGKPMV IQFSRPGGLT KKLFFASHFH KNFIFNNEHH YYPPPPPPSR MVKSDILMYK 

       310        320        330        340        350        360 
QQQKKKKKKY VKKNLGDPYF MINENAITGG EFRDGRTTVM IKNIPNKYTQ KLLLKMLDTH 

       370        380        390        400        410        420 
CKDCNQSVIK EGNKTPMSSY DFVYLPIDFS NKSNVGYGFV NMTSPEAVWR LYKSFHNQHW 

       430        440        450        460        470        480 
RDFTTTRKIC EVTYARIQGL ESLREHFKNV RLAGVEIDEY MPVVFSPPRD GRLSPEPVAI 

       490        500        510        520 
VDPWDKPVDD EDRCCKSRDG FVVSDKKMVG SDGCGFCLSE RIENGGV 

References and documentation are available.

Number of amino acids: 527

Molecular weight: 60374.32

Theoretical pI: 9.36

Amino acid composition: 
Ala (A)  19	  3.6%
Arg (R)  31	  5.9%
Asn (N)  29	  5.5%
Asp (D)  21	  4.0%
Cys (C)   8	  1.5%
Gln (Q)  25	  4.7%
Glu (E)  27	  5.1%
Gly (G)  29	  5.5%
His (H)  20	  3.8%
Ile (I)  23	  4.4%
Leu (L)  33	  6.3%
Lys (K)  33	  6.3%
Met (M)  17	  3.2%
Phe (F)  36	  6.8%
Pro (P)  54	 10.2%
Ser (S)  33	  6.3%
Thr (T)  27	  5.1%
Trp (W)   4	  0.8%
Tyr (Y)  17	  3.2%
Val (V)  41	  7.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 48
Total number of positively charged residues (Arg + Lys): 64

Atomic composition:

Carbon      C	      2727
Hydrogen    H	      4187
Nitrogen    N	       751
Oxygen      O	       755
Sulfur      S	        25

Formula: C2727H4187N751O755S25
Total number of atoms: 8445

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    47830
Abs 0.1% (=1 g/l)   0.792, assuming all pairs of Cys residues form cystines


Ext. coefficient    47330
Abs 0.1% (=1 g/l)   0.784, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.88
This classifies the protein as unstable.



Aliphatic index: 67.61

Grand average of hydropathicity (GRAVY): -0.512