ProtParam
User-provided sequence:
10 20 30 40 50 60
MSVTGTEIDS DTAKNILSVD ELQNYGINAS DLQKLKSGGI YTVNTVLSTT RRHLCKIKGL
70 80 90 100 110 120
SEVKVEKIKE AAGKIIQVGF IPATVQLDIR QRVYSLSTGS KQLDSILGGG IMTMSITEVF
130 140 150 160 170 180
GEFRCGKTQM SHTLCVTTQL PREMGGGEGK VAYIDTEGTF RPERIKQIAE GYELDPESCL
190 200 210 220 230 240
ANVSYARALN SEHQMELVEQ LGEELSSGDY RLIVVDSIMA NFRVDYCGRG ELSERQQKLN
250 260 270 280 290 300
QHLFKLNRLA EEFNVAVFLT NQVQSDPGAS ALFASADGRK PIGGHVLAHA SATRILLRKG
310 320 330
RGDERVAKLQ DSPDMPEKEC VYVIGEKGIT DSSD
References and
documentation are available.
Number of amino acids: 334
Molecular weight: 36612.57
Theoretical pI: 5.66
Total number of negatively charged residues (Asp + Glu): 45
Total number of positively charged residues (Arg + Lys): 39
Atomic composition:
Carbon C 1590
Hydrogen H 2585
Nitrogen N 451
Oxygen O 509
Sulfur S 14
Formula: C
1590H
2585N
451O
509S
14
Total number of atoms: 5149
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 13785
Abs 0.1% (=1 g/l) 0.377, assuming all pairs of Cys residues form cystines
Ext. coefficient 13410
Abs 0.1% (=1 g/l) 0.366, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 32.16
This classifies the protein as stable.
Aliphatic index: 89.01
Grand average of hydropathicity (GRAVY): -0.299