ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSVTIEAALK AATEQLQQSG SDSPALDAAV LLCHVLAKPR SYLLTWPDKI LEKPTLASLE 

        70         80         90        100        110        120 
LLLARRRAGE PMAYILGERE FWSLPLKVSP STLIPRPDTE RLVELALDKA ALIDGELLDL 

       130        140        150        160        170        180 
GTGTGAIALA LASELPTRQV TGIDLRPEAA ELARENATRL AIHNAQFFQG SWFSPLADGT 

       190        200        210        220        230        240 
KFALIVSNPP YIEENDPHLS LGDVRFEPKS ALVAAENGLA DIRHISTHAP HFLLDGGWLL 

       250        260        270        280 
FEHGYDQGVA VRTILRDLGY QNIITEQDYA GHDRVTLGQY KTEREA 

References and documentation are available.

Number of amino acids: 286

Molecular weight: 31317.66

Theoretical pI: 5.06

Amino acid composition: 
Ala (A)  35	 12.2%
Arg (R)  17	  5.9%
Asn (N)   6	  2.1%
Asp (D)  17	  5.9%
Cys (C)   1	  0.3%
Gln (Q)  10	  3.5%
Glu (E)  22	  7.7%
Gly (G)  19	  6.6%
His (H)   8	  2.8%
Ile (I)  15	  5.2%
Leu (L)  44	 15.4%
Lys (K)   9	  3.1%
Met (M)   2	  0.7%
Phe (F)   8	  2.8%
Pro (P)  17	  5.9%
Ser (S)  16	  5.6%
Thr (T)  17	  5.9%
Trp (W)   4	  1.4%
Tyr (Y)   7	  2.4%
Val (V)  12	  4.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 39
Total number of positively charged residues (Arg + Lys): 26

Atomic composition:

Carbon      C	      1406
Hydrogen    H	      2230
Nitrogen    N	       382
Oxygen      O	       421
Sulfur      S	         3

Formula: C1406H2230N382O421S3
Total number of atoms: 4442

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    32430
Abs 0.1% (=1 g/l)   1.036, assuming all pairs of Cys residues form cystines


Ext. coefficient    32430
Abs 0.1% (=1 g/l)   1.036, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.20
This classifies the protein as unstable.



Aliphatic index: 104.86

Grand average of hydropathicity (GRAVY): -0.088