ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSVTSREQKF SGKYSSYTAQ DRQGLVNAVT CVLSSSSDPV AVSSDYSNSL SIAREVNAYA 

        70         80         90        100        110        120 
KIAGCDWTYY VQKLEVTIGR NTDSLNLNAV PGTVVKKNID IDLGPAKIVS RKHAAIRFNL 

       130        140        150        160        170        180 
ESGSWELQIF GRNGAKVNFR RIPTGPDSPP TVLQSGCIID IGGVQMIFIL PEQETIISDY 

       190        200        210        220        230        240 
CLNHLMPKLL STYGTNGNNN PLLRNIIEGS TYLREQRLQE EARLQQLDHL HTPLSSSSDV 

       250        260        270        280        290        300 
NPIGDPHGDT IMMEEDEEDE NYTRGGIRPN TYTSSSNNAV TNGNVPHIEN PSDLSLDENR 

       310        320        330        340        350        360 
YIKPPQSYAS MITQAILSTP EGSISLADIY KFISDNYAFY RFSQMAWQNS VRHNLSLNKA 

       370        380        390        400        410        420 
FEKVPKRAGQ QGKGMNWKIS DEVRRDFLNK WNAGKLSKIR RGASVTRQLQ LHMSKFGEIP 

       430        440        450        460        470        480 
APESSSIDPR GIKAQKVKKS LQATSSILGE SAPQLQRTQL TGQISTTTSM DVTTNANVNN 


SSLS 

References and documentation are available.

Number of amino acids: 484

Molecular weight: 53490.02

Theoretical pI: 8.46

Amino acid composition: 
Ala (A)  28	  5.8%
Arg (R)  26	  5.4%
Asn (N)  36	  7.4%
Asp (D)  24	  5.0%
Cys (C)   4	  0.8%
Gln (Q)  26	  5.4%
Glu (E)  24	  5.0%
Gly (G)  31	  6.4%
His (H)   8	  1.7%
Ile (I)  35	  7.2%
Leu (L)  38	  7.9%
Lys (K)  25	  5.2%
Met (M)  10	  2.1%
Phe (F)  11	  2.3%
Pro (P)  24	  5.0%
Ser (S)  57	 11.8%
Thr (T)  30	  6.2%
Trp (W)   5	  1.0%
Tyr (Y)  16	  3.3%
Val (V)  26	  5.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 48
Total number of positively charged residues (Arg + Lys): 51

Atomic composition:

Carbon      C	      2329
Hydrogen    H	      3718
Nitrogen    N	       670
Oxygen      O	       746
Sulfur      S	        14

Formula: C2329H3718N670O746S14
Total number of atoms: 7477

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    51590
Abs 0.1% (=1 g/l)   0.964, assuming all pairs of Cys residues form cystines


Ext. coefficient    51340
Abs 0.1% (=1 g/l)   0.960, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 47.27
This classifies the protein as unstable.



Aliphatic index: 80.19

Grand average of hydropathicity (GRAVY): -0.509