ProtParam
User-provided sequence:
10 20 30 40 50 60
MSVVTTTSMQ SDSKKRVQLM GDTTSPFSLD TPNPKLMFTP LDLPTTAELM QRCLAVNPFE
70 80 90 100 110 120
AKFREANQKI SSGSMQPNTS GANQSLEALE ANGGSQFSGS NAGTMSDLLL KIPQASLQHS
130 140 150 160 170 180
PGIFSNMLLN AGDSEGTTRE NLKTADISKL LSVAGDFSAQ APRTADVLNA VLDMHSDRLH
190 200 210 220 230 240
TINYLNNKPD FSALLRSPSS SAPNSASVLT NAMAIPSTSG APFPGTTLLV PPKTVSSYHS
250 260 270 280 290 300
PLGASSQPPS TQKSPADGSW DHINGEKQIK KEIPYFNDDA MMLMERSNMS SSGSDQDQSA
310 320 330 340 350 360
DMSNAGSTAS TSTGNPVGRP QNGTPGRGRG RGRSTTADMQ PDERRNTILE RNKAAAVRYR
370 380 390 400 410 420
KRKKEEHDDM MGRVQAMEAE KNQLLAIQTQ NQVLRRELER VTALLTERES RCVCLKGVPM
430 440 450 460
SDEQHADHHH RNTNGMYSGS DMLNGLGQIN GMQLKLPKLQ
References and
documentation are available.
Number of amino acids: 460
Molecular weight: 49722.67
Theoretical pI: 7.77
Total number of negatively charged residues (Asp + Glu): 47
Total number of positively charged residues (Arg + Lys): 48
Atomic composition:
Carbon C 2103
Hydrogen H 3417
Nitrogen N 637
Oxygen O 704
Sulfur S 26
Formula: C
2103H
3417N
637O
704S
26
Total number of atoms: 6887
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 13075
Abs 0.1% (=1 g/l) 0.263, assuming all pairs of Cys residues form cystines
Ext. coefficient 12950
Abs 0.1% (=1 g/l) 0.260, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 41.62
This classifies the protein as unstable.
Aliphatic index: 64.54
Grand average of hydropathicity (GRAVY): -0.648