ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSWARSHLCS ALSLAAVSAR GATMEGAAYR WLSAWPAPQE PSMEYQDAVR TLNTLQTNAS 

        70         80         90        100        110        120 
CLEEVKRQRS DPQAQLEAME VYLARSGLQV EDLNQLKIIH VTGTKGKGST CAFTERILRN 

       130        140        150        160        170        180 
YGLKTGFFSS PHLVQVRERI RINGKPISPE LFTKYFWHLY QQLEEFKDDS HISMPSYFRF 

       190        200        210        220        230        240 
LTLMAFHVFL QEKVDLAVVE VGIGGAFDCT NIIRKPVVCG VSSLGIDHTS LLGDTVEKIA 

       250        260        270        280        290        300 
WQKGGIFKPG VPAFTVLQPE GPLAVLRDRA QQISCPLYLC PPLEALEQVG LPLSLGLEGE 

       310        320        330        340        350        360 
HQRHNAALAL QLARCWLEQQ NYHDIQELKA SKPSIRWQLP LAPVFRPTPH MRRGLRDTEW 

       370        380        390        400        410        420 
PGRTQVLQRG PVTWYLDGAH TTSSVQACVH WYCQSLERSR RTDGGHEVHI LLFNSTGDRD 

       430        440        450        460        470        480 
SAALLKLLQP CHFDYAVFCP NVTEISSIGN ADQQNFTVTL DQVLLRCLQH QKHWNCLAEK 

       490        500        510        520        530        540 
QASSNLWGSC GPESARPGSL LLAPHPPQPT STSSLVFSCI SHALQWISQG RDPIFQPESP 

       550        560        570        580 
PRNLLNHPTA NSGASILREA AAIHVLVTGS LHLVGGALRL LDPSMSQ 

References and documentation are available.

Number of amino acids: 587

Molecular weight: 65269.64

Theoretical pI: 7.61

Amino acid composition: 
Ala (A)  47	  8.0%
Arg (R)  35	  6.0%
Asn (N)  17	  2.9%
Asp (D)  21	  3.6%
Cys (C)  16	  2.7%
Gln (Q)  39	  6.6%
Glu (E)  31	  5.3%
Gly (G)  38	  6.5%
His (H)  23	  3.9%
Ile (I)  24	  4.1%
Leu (L)  73	 12.4%
Lys (K)  18	  3.1%
Met (M)   8	  1.4%
Phe (F)  20	  3.4%
Pro (P)  38	  6.5%
Ser (S)  49	  8.3%
Thr (T)  30	  5.1%
Trp (W)  13	  2.2%
Tyr (Y)  12	  2.0%
Val (V)  35	  6.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 52
Total number of positively charged residues (Arg + Lys): 53

Atomic composition:

Carbon      C	      2908
Hydrogen    H	      4557
Nitrogen    N	       825
Oxygen      O	       839
Sulfur      S	        24

Formula: C2908H4557N825O839S24
Total number of atoms: 9153

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    90380
Abs 0.1% (=1 g/l)   1.385, assuming all pairs of Cys residues form cystines


Ext. coefficient    89380
Abs 0.1% (=1 g/l)   1.369, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 52.64
This classifies the protein as unstable.



Aliphatic index: 89.74

Grand average of hydropathicity (GRAVY): -0.195