ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSWHEQDYGF GTSSENSKIN SDEFEDSMDV TEFNNPGEES TYPQANSWND SNKTKDIAEY 

        70         80         90        100        110        120 
YDYSWSGQRE ANQQIPQPVP VQPRYPDEQN FSMNGETYPS EAYDYDYSGQ EEAINAMNIL 

       130        140        150        160        170        180 
QGNLERSNEY YSEGVPYSEE DLGAYAGGMT EDDQMYSNFN ETGEFNLDIG SSKFSYLNAK 

       190        200        210        220        230        240 
NPYPAWIAEN SIPITAENIL EIFQELQAKF GFQYDSMLNM YDFFMVLLDS RSSRMDAENA 

       250        260        270        280        290        300 
LKSLHADYIG GRNANYRKWY FSSSMDIDDS VGLQNCKFSS NGPKIKQAKK KQKRKSNKAE 

       310        320        330        340        350        360 
TEGTNEPETS VQIDPLNVSM ENWENEMKNL DCETQVRQLA LYLLCWGEAN NIRFCPECLC 

       370        380        390        400        410        420 
FIFKLANDFM QSEDYAKSEP IEDDCFYLDN VITPLYEFIR DQQFELLDGK LVRRERDHAQ 

       430        440        450        460        470        480 
IIGYDDINQL FWYPEGIARI VTVDGTQLIT LPKWERFHKL SEVDWKKAFY KTFYESRSWF 

       490        500        510        520        530        540 
HLVTNFNRIW VIHFTTYWYY TVFNSPTIIE KNFRQSVGPK PIPSCHWTSV SLGGAVATLL 

       550        560        570        580        590        600 
MLLATIFEWI HVPRKFPGSR PLLKRFLILI LFFILNVAPT VFVFGFSTEE QQRTTGRLTV 

       610        620        630        640        650        660 
AIVHFIFSVF TFIYFSLVPL NNLFHRAYKS SSRTHLANRY FTADYARLQI NDMCVSWGLW 

       670        680        690        700        710        720 
LLVFGAKFTE SYFFLSLSFR DPILVLSTMK PYLCNITFLG SHLCIWQPKI LLGIMYVTDL 

       730        740        750        760        770        780 
VLFFLDTYLW YILVNTVFSV ARSFFLGISI WTPWRNIFAR MPKRIYSKIL CTPEVDSSYK 

       790        800        810        820        830        840 
PKVLVSQIWN SIIISLYREH LLAIEHVQRL IYHQVNSLDG DGSKTLKTPT FFVSQEDSSF 

       850        860        870        880        890        900 
NTEYFPAHSE AERRLSFFAQ SLATPIPEPI PVDAMPTFTV LVPHYGEKIL LSLKEIIREQ 

       910        920        930        940        950        960 
DKLSRVTLLE YLKQLHANEW KCFVRDTKIL AEEDALSNQD LNSQDESMKA EQLHKKFDDL 

       970        980        990       1000       1010       1020 
PFYCIGFKNA TPEYTLRTRI WASLRSQTLY RTVSGFMNYS RAIKLLYRVE NPDVAQLFEG 

      1030       1040       1050       1060       1070       1080 
QMDVLEYELD RMASRKFKMC VSMQRYAKFT ADEIENTEFI LRAYPDLLIA YLDEDPPKEG 

      1090       1100       1110       1120       1130       1140 
ETTPQLYAAL IDGYSELDEN KKRKPKYRIK LSGNPILGDG KSDNQNLSLP FYRGEYIQLI 

      1150       1160       1170       1180       1190       1200 
DANQDNYLEE CLKIRSILAE FEAFDLKTND PYAETNALYQ NNPVAIMGAR EYIFSENIGI 

      1210       1220       1230       1240       1250       1260 
LGDVAAGKEQ TFGTLFARTM AQIGGKLHYG HPDFLNAIYM TTRGGVSKAQ KGLHVNEDIY 

      1270       1280       1290       1300       1310       1320 
AGMTALQRGG RIKHCEYYQC GKGRDLGFGS ILNFTTKIGT GMGEQMVSRE YYYLGTQLPF 

      1330       1340       1350       1360       1370       1380 
DRFLSFYYAH PGFHINNIFI MLSVQLFMVV LVNLGGMYHV VTVCDYDHDQ KLTVPMRPEG 

      1390       1400       1410       1420       1430       1440 
CYQLNPVVNW LKRCIISIFI VFFISFVPLT VQELTERGAW RALTRLGKHF ASFSPMFEVF 

      1450       1460       1470       1480       1490       1500 
ACQTYAQSVI ANLSFGGARY IGTGRGFATA RLSFSLLFSR FAGPSIYLGS RTLLMLLFGT 

      1510       1520       1530       1540       1550       1560 
MTVWIPHLIY FWISTLAMCI SPFIFNPHQF SWTDFFVDYR EFIRWLSRGN SRSHINSWIG 

      1570       1580       1590       1600       1610       1620 
YCRLTRTRIT GYKRRLLGVP VSKGVIDTSR AHFTNMFFTE IFIPLMLVPL TLVSYFFIDS 

      1630       1640       1650       1660       1670       1680 
QPGNPDPSKV TNPILRILIL AFLPIIVAAV VSMTFAGMAC MMGPLLDLCC KKFGAVLAAL 

      1690       1700       1710       1720       1730       1740 
AHGITVFMFI IVFEVSWYLE AWCLAKTVLS MLCIIAIQRF FFKIIQVLFL TRELKHDGTN 

      1750       1760       1770       1780       1790       1800 
LAWWTGKWYS RGLGFHALSQ PSRELICKMT ELNFFAADFF LCHLLLFLML PVLLIPFIDR 

      1810       1820       1830       1840       1850       1860 
WHSMMLFWLK PSKQIRPPIY SMRQNRLRKK IVQRYGTMFF LLLIAFLALI IIPLVVAKDL 

      1870       1880       1890 
LANFEMDILR TYGLMQPRDT NWTENGTNWT TVNE 

References and documentation are available.

Number of amino acids: 1894

Molecular weight: 219183.33

Theoretical pI: 6.29

Amino acid composition: 
Ala (A) 107	  5.6%
Arg (R)  95	  5.0%
Asn (N)  94	  5.0%
Asp (D)  88	  4.6%
Cys (C)  31	  1.6%
Gln (Q)  72	  3.8%
Glu (E) 109	  5.8%
Gly (G) 100	  5.3%
His (H)  37	  2.0%
Ile (I) 134	  7.1%
Leu (L) 198	 10.5%
Lys (K)  88	  4.6%
Met (M)  56	  3.0%
Phe (F) 137	  7.2%
Pro (P)  85	  4.5%
Ser (S) 130	  6.9%
Thr (T) 106	  5.6%
Trp (W)  40	  2.1%
Tyr (Y)  93	  4.9%
Val (V)  94	  5.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 197
Total number of positively charged residues (Arg + Lys): 183

Atomic composition:

Carbon      C	     10058
Hydrogen    H	     15249
Nitrogen    N	      2547
Oxygen      O	      2784
Sulfur      S	        87

Formula: C10058H15249N2547O2784S87
Total number of atoms: 30725

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   360445
Abs 0.1% (=1 g/l)   1.644, assuming all pairs of Cys residues form cystines


Ext. coefficient   358570
Abs 0.1% (=1 g/l)   1.636, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.99
This classifies the protein as stable.



Aliphatic index: 88.41

Grand average of hydropathicity (GRAVY): -0.085