ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSWLFGDKTP TDDANAAVGG QDTTKPKELS LKQSLGFEPN INNIISGPGG MHVDTARLHP 

        70         80         90        100        110        120 
LAGLDKGVEY LDLEEEQLSS LEGSQGLIPS RGWTDDLCYG TGAVYLLGLG IGGFSGMMQG 

       130        140        150        160        170        180 
LQNIPPNSPG KLQLNTVLNH ITKRGPFLGN NAGILALSYN IINSTIDALR GKHDTAGSIG 

       190        200        210        220 
AGALTGALFK SSKGLKPMGY SSAMVAAACA VWCSVKKRLL EK 

References and documentation are available.

Number of amino acids: 222

Molecular weight: 23243.56

Theoretical pI: 6.90

Amino acid composition: 
Ala (A)  18	  8.1%
Arg (R)   5	  2.3%
Asn (N)  12	  5.4%
Asp (D)  11	  5.0%
Cys (C)   3	  1.4%
Gln (Q)   7	  3.2%
Glu (E)   8	  3.6%
Gly (G)  31	 14.0%
His (H)   4	  1.8%
Ile (I)  12	  5.4%
Leu (L)  29	 13.1%
Lys (K)  14	  6.3%
Met (M)   6	  2.7%
Phe (F)   5	  2.3%
Pro (P)  11	  5.0%
Ser (S)  18	  8.1%
Thr (T)  12	  5.4%
Trp (W)   3	  1.4%
Tyr (Y)   5	  2.3%
Val (V)   8	  3.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 19
Total number of positively charged residues (Arg + Lys): 19

Atomic composition:

Carbon      C	      1026
Hydrogen    H	      1643
Nitrogen    N	       281
Oxygen      O	       315
Sulfur      S	         9

Formula: C1026H1643N281O315S9
Total number of atoms: 3274

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    24075
Abs 0.1% (=1 g/l)   1.036, assuming all pairs of Cys residues form cystines


Ext. coefficient    23950
Abs 0.1% (=1 g/l)   1.030, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.93
This classifies the protein as stable.



Aliphatic index: 90.59

Grand average of hydropathicity (GRAVY): -0.098