ProtParam
User-provided sequence:
10 20 30 40 50 60
MSYAYLFKYI IIGDTGVGKS CLLLQFTDKR FQPVHDLTIG VEFGARMITI DNKPIKLQIW
70 80 90 100 110 120
DTAGQESFRS ITRSYYRGAA GALLVYDITR RETFNHLASW LEDARQHANA NMTIMLIGNK
130 140 150 160 170 180
CDLAHRRAVS TEEGEQFAKE HGLIFMEASA KTAQNVEEAF IKTAATIYKK IQDGVFDVSN
190 200 210
ESYGIKVGYG GIPGPSGGRD GSTSQGGGCC G
References and
documentation are available.
Number of amino acids: 211
Molecular weight: 23164.26
Theoretical pI: 6.96
Total number of negatively charged residues (Asp + Glu): 23
Total number of positively charged residues (Arg + Lys): 23
Atomic composition:
Carbon C 1026
Hydrogen H 1602
Nitrogen N 284
Oxygen O 310
Sulfur S 9
Formula: C
1026H
1602N
284O
310S
9
Total number of atoms: 3231
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 24660
Abs 0.1% (=1 g/l) 1.065, assuming all pairs of Cys residues form cystines
Ext. coefficient 24410
Abs 0.1% (=1 g/l) 1.054, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 38.15
This classifies the protein as stable.
Aliphatic index: 78.67
Grand average of hydropathicity (GRAVY): -0.230