ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSYEKKSVEG AYIDDSTTFE AFHLDSRLLQ AIKNIGFQYP TLIQSHAIPL ALQQKRDIIA 

        70         80         90        100        110        120 
KAATGSGKTL AYLIPVIETI LEYKKTIDNG EENGTLGIIL VPTRELAQQV YNVLEKLVLY 

       130        140        150        160        170        180 
CSKDIRTLNI SSDMSDSVLS TLLMDQPEII VGTPGKLLDL LQTKINSISL NELKFLVVDE 

       190        200        210        220        230        240 
VDLVLTFGYQ DDLNKIGEYL PLKKNLQTFL MSATLNDDIQ ALKQKFCRSP AILKFNDEEI 

       250        260        270        280        290        300 
NKNQNKLLQY YVKVSEFDKF LLCYVIFKLN LIKGKTLIFV NNIDRGYRLK LVMEQFGIKS 

       310        320        330        340        350        360 
CILNSELPVN SRQHIVDQFN KNVYQLLIAT DDTEYIKEED DEIEEGHNTE NQEEKSLEGE 

       370        380        390        400        410        420 
PENDKKPSKK KKVQVKKDKE YGVSRGVDFK NVACVLNFDL PTTAKSYVHR VGRTARGGKT 

       430        440        450        460        470        480 
GTAISFVVPL KEFGKHKPSM LQTAKKDERI LSRIIKQQSK LGLELQPYKF DQKQVEAFRY 

       490        500        510        520        530        540 
RMEDGFRAVT QVAIREARVK ELKQELLASE KLKRHFEENP KELQSLRHDK ELHPARVQQH 

       550        560        570        580        590 
LKRVPDYLLP ESARGNGTKV KFVPFHNAKK RHSHKKGRVS KPKNGKVDPL KNFK 

References and documentation are available.

Number of amino acids: 594

Molecular weight: 68059.58

Theoretical pI: 9.22

Amino acid composition: 
Ala (A)  27	  4.5%
Arg (R)  26	  4.4%
Asn (N)  31	  5.2%
Asp (D)  34	  5.7%
Cys (C)   5	  0.8%
Gln (Q)  32	  5.4%
Glu (E)  45	  7.6%
Gly (G)  29	  4.9%
His (H)  13	  2.2%
Ile (I)  38	  6.4%
Leu (L)  69	 11.6%
Lys (K)  70	 11.8%
Met (M)   7	  1.2%
Phe (F)  25	  4.2%
Pro (P)  22	  3.7%
Ser (S)  33	  5.6%
Thr (T)  28	  4.7%
Trp (W)   0	  0.0%
Tyr (Y)  20	  3.4%
Val (V)  40	  6.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 79
Total number of positively charged residues (Arg + Lys): 96

Atomic composition:

Carbon      C	      3056
Hydrogen    H	      4939
Nitrogen    N	       831
Oxygen      O	       897
Sulfur      S	        12

Formula: C3056H4939N831O897S12
Total number of atoms: 9735

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    30050
Abs 0.1% (=1 g/l)   0.442, assuming all pairs of Cys residues form cystines


Ext. coefficient    29800
Abs 0.1% (=1 g/l)   0.438, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.77
This classifies the protein as stable.



Aliphatic index: 94.33

Grand average of hydropathicity (GRAVY): -0.508