ProtParam
User-provided sequence:
10 20 30 40 50 60
MSYGLTGTSS KLRGTSSIFS WTQVRHFSRR RIAYPFYPFK KLGRQHPKKH DTNLKTAMRQ
70 80 90 100 110 120
FLGPKNYKGE YVMNKYFTVP TNHVPNYIKP DLERGQSLEH PVTKKPLQLR YDGTLGPPPV
130 140 150 160 170 180
ENKRLQNIFK DRLLQPFPSN PHCKTNYVLS PQLKQSIFEE ITVEGLSAQQ VSQKYGLKIP
190 200 210 220 230 240
RVEAIVKLVS VENSWNRRNR VSSDLKTMDE TLYRMFPVFD SDASFKRENL SEIPVPQKTL
250 260 270 280 290 300
ASRFLTIAES EPFGPVDAAH VLELEPAVET LRNLSTVGEH SSGHQQSTNK NTKVIYGELV
310 320 330 340
EGERSQYKFT NAKVGKVGYR YGSGNRDNKK DRRIGFNKLG QMVYI
References and
documentation are available.
Number of amino acids: 345
Molecular weight: 39575.17
Theoretical pI: 9.88
Total number of negatively charged residues (Asp + Glu): 32
Total number of positively charged residues (Arg + Lys): 54
Atomic composition:
Carbon C 1772
Hydrogen H 2794
Nitrogen N 504
Oxygen O 512
Sulfur S 7
Formula: C
1772H
2794N
504O
512S
7
Total number of atoms: 5589
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 34840
Abs 0.1% (=1 g/l) 0.880, assuming all pairs of Cys residues form cystines
Ext. coefficient 34840
Abs 0.1% (=1 g/l) 0.880, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 44.68
This classifies the protein as unstable.
Aliphatic index: 71.68
Grand average of hydropathicity (GRAVY): -0.720